Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 76745..77304 Replicon plasmid p2RM8082
Accession NZ_CP043828
Organism Escherichia coli strain RM8082

Toxin (Protein)


Gene name relE Uniprot ID A0A733EV64
Locus tag F1745_RS26755 Protein ID WP_001582571.1
Coordinates 76745..77020 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag F1745_RS26760 Protein ID WP_001178089.1
Coordinates 77020..77304 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1745_RS26730 (73049) 73049..74395 - 1347 WP_001132032.1 protein YagA -
F1745_RS26735 (74748) 74748..75350 + 603 WP_000517695.1 ProQ/FINO family protein -
F1745_RS26740 (75406) 75406..75900 + 495 WP_097481107.1 hypothetical protein -
F1745_RS26745 (75900) 75900..76166 + 267 WP_000627829.1 hypothetical protein -
F1745_RS26750 (76436) 76436..76639 - 204 Protein_85 hypothetical protein -
F1745_RS26755 (76745) 76745..77020 - 276 WP_001582571.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F1745_RS26760 (77020) 77020..77304 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
F1745_RS26765 (78425) 78425..79456 - 1032 WP_000907861.1 plasmid replication initiator RepA -
F1745_RS26770 (80462) 80462..80692 + 231 WP_228772074.1 ash family protein -
F1745_RS26775 (80610) 80610..80897 + 288 WP_000074849.1 conjugal transfer protein TraA -
F1745_RS26780 (81339) 81339..81872 + 534 WP_001306770.1 transcription termination/antitermination NusG family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..97743 97743


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10807.48 Da        Isoelectric Point: 9.5296

>T139337 WP_001582571.1 NZ_CP043828:c77020-76745 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNDQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T139337 NZ_CP058651:902372-902779 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT139337 WP_001178089.1 NZ_CP043828:c77304-77020 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT139337 NZ_CP043828:c77304-77020 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A733EV64

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References