Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 25224..25868 Replicon plasmid p4RM8082
Accession NZ_CP043826
Organism Escherichia coli strain RM8082

Toxin (Protein)


Gene name hicA Uniprot ID A0A7L6LII1
Locus tag F1745_RS25715 Protein ID WP_000833471.1
Coordinates 25224..25406 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A7L6LHN8
Locus tag F1745_RS25720 Protein ID WP_000466317.1
Coordinates 25431..25868 (+) Length 146 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1745_RS25680 (F1745_25855) 22229..22552 + 324 WP_064565519.1 hypothetical protein -
F1745_RS25685 (F1745_25860) 22593..22817 + 225 WP_064565522.1 hypothetical protein -
F1745_RS25690 (F1745_25865) 22913..23137 + 225 WP_000749828.1 hypothetical protein -
F1745_RS25695 (F1745_25870) 23188..23454 + 267 WP_000868277.1 hypothetical protein -
F1745_RS25700 (F1745_25875) 23444..23686 + 243 WP_001450803.1 hypothetical protein -
F1745_RS25705 (F1745_25885) 24126..24455 + 330 WP_021558913.1 hypothetical protein -
F1745_RS25710 (F1745_25890) 24513..24791 + 279 WP_064565525.1 hypothetical protein -
F1745_RS25715 (F1745_25895) 25224..25406 + 183 WP_000833471.1 type II toxin-antitoxin system HicA family toxin Toxin
F1745_RS25720 (F1745_25900) 25431..25868 + 438 WP_000466317.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
F1745_RS25725 (F1745_25905) 25897..26601 - 705 WP_032274884.1 hypothetical protein -
F1745_RS25730 (F1745_25910) 26598..27359 - 762 WP_001097938.1 conjugal transfer protein TraL -
F1745_RS25735 (F1745_25915) 27369..27776 - 408 WP_001190621.1 hypothetical protein -
F1745_RS25740 (F1745_25920) 28122..28457 + 336 WP_000963832.1 DUF6290 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..37652 37652


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-60)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(7-138)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6675.97 Da        Isoelectric Point: 11.0013

>T139326 WP_000833471.1 NZ_CP043826:25224-25406 [Escherichia coli]
MKSADLLKELIAAGCELKRHKASSHQIWWSPITGKTFPVPHPKKDLPLGTVRSIRKMAGI

Download         Length: 183 bp

>T139326 NZ_CP058644:c2451207-2450956 [Pseudomonas yamanorum]
GTGTCGAAAAATGAAAAGCTGCTGGCCAAACTGCTCAATGAGCAAATGGCCTTTACCTGGCCCGAACTCGTCACGCTGCT
GCGTCGACTGGGCTACACACAAATCGAAGGGGCCGGGAGCCGCGTCAAGTTCGACAAGGGCGATCCGAGCGCAATGATCA
CCCTGCACAAACCGCATCCCGGCAACGAGTTGAAACACTACATTCGGCGCCAGATCATCGAACAATTGAAATCAGGAGAC
CTGATTCAGTGA

Antitoxin


Download         Length: 146 a.a.        Molecular weight: 16227.10 Da        Isoelectric Point: 4.3135

>AT139326 WP_000466317.1 NZ_CP043826:25431-25868 [Escherichia coli]
MFFSVGVETPKDDHTAYGITVPAFDRFDFGCVSAADTQSEIPVMAREAILAIVEEMVLSGSYSVDDIHDDGCLTYAANQD
YSHCDSWFVIDVDLSEIEGKQQRINIALPDVLIRRIDGFVRESGGVYRDRSHFLAQAARHELSYK

Download         Length: 438 bp

>AT139326 NZ_CP058644:c2450959-2450603 [Pseudomonas yamanorum]
GTGAACAACCAGTTGAAACACAACGGCTATATCGGCTCGATCGAAGCCAGCATCGAAGACAATTGCCTGTTCGGAAAGCT
TCTATTCATCAAGGCGCTCGTCAGCTATGAAGGCAAAACCGTCGCCGAACTGGAAGCCGCCTTTCGCGACGCGGTGGACG
ACTACCTCGACACTTGCCACAGCCTCGGCCAAACACCCGAAAAACCTTGCAAGGGCTCGTTCAACGTTCGGGTCGGCCAT
GACCTGCATTTGGCCGCGGCCTTGGCCGCGACCCGACAGAAAGTGACACTGAACGACCTGACGCGACAGGCGCTGAACGA
GTTCCTGCAACATCACGGTGCGCTCGTAACGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6LII1

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6LHN8

References