Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4843239..4843736 Replicon chromosome
Accession NZ_CP043825
Organism Escherichia coli strain RM8082

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag F1745_RS23945 Protein ID WP_001173343.1
Coordinates 4843563..4843736 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag F1745_RS23940 Protein ID WP_000398619.1
Coordinates 4843239..4843535 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1745_RS23930 (4838598) 4838598..4842527 + 3930 WP_001542592.1 autotransporter outer membrane beta-barrel domain-containing protein -
F1745_RS23935 (4842740) 4842740..4843012 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
F1745_RS23940 (4843239) 4843239..4843535 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
F1745_RS23945 (4843563) 4843563..4843736 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
F1745_RS23950 (4843855) 4843855..4845372 - 1518 WP_001295087.1 lysine--tRNA ligase -
F1745_RS23955 (4845609) 4845609..4847066 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4819929..4852589 32660


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T139323 WP_001173343.1 NZ_CP043825:4843563-4843736 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T139323 NZ_CP058639:670859-671119 [Rhodococcus sp. ZPP]
ATGAAACTTGTCTGGGACGAGTCCGCGTGGGAGGACTACGTCTGGTGGCAGGCGCAGGATCGGAAGATCGTCAAGCGGAT
CAACACACTACTCAAGGATGTCGAACGCAACGGGAACGACGGAATCGGCAAGCCCGAGCCCCTCAAGCACGGTTTTCAAG
GCTACTGGTCCCGGCGTATCACCGATGAGCACCGGCTGGTCTATAAAATTGTCGATGATCAGATCCGCATTGCTGCCTGC
CGTTACCACTACGGACACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT139323 WP_000398619.1 NZ_CP043825:4843239-4843535 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT139323 NZ_CP058639:670611-670862 [Rhodococcus sp. ZPP]
ATGCGAACTATGACCTACTCGGAGTCTCGCGCGCGGTACGCCGAGGTCCTCAACGCCGTCGTCGATGACCGGGAAGAAAT
CGTCATCACGCGGGCGGGTCACGAGCCGGTGGTGATGGTTTCGCTCGACGACTATGAGTCGTTGAAGGAAACGGCATACT
TGCTCAGGAGTCCGGAGAACGCCCGCCGGCTGTTGGCGTCGATCGATCGGTTGGAGTCCGGCGGGGGAACTGTCCGGGAT
CTGGCTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References