Detailed information of TA system TA139321

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4223141..4223939
• Replicon: chromosome
• Accession: NZ_CP043825
• Organism: Escherichia coli strain RM8082

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: F1745_RS20935
• Protein ID: WP_096848640.1
• Coordinates: 4223562..4223939 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: F1745_RS20930
• Protein ID: WP_096848639.1
• Coordinates: 4223141..4223515 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1745_RS20895 (4219050)	4219050..4219730	+	681	WP_001282919.1	WYL domain-containing protein	-
F1745_RS20900 (4219878)	4219878..4220555	+	678	WP_001097301.1	hypothetical protein	-
F1745_RS20905 (4220561)	4220561..4220794	+	234	WP_001278282.1	DUF905 family protein	-
F1745_RS20910 (4220884)	4220884..4221699	+	816	Protein_4097	DUF932 domain-containing protein	-
F1745_RS20915 (4221794)	4221794..4222279	+	486	WP_000206667.1	antirestriction protein	-
F1745_RS20920 (4222295)	4222295..4222771	+	477	WP_053289525.1	RadC family protein	-
F1745_RS20925 (4222840)	4222840..4223061	+	222	WP_000692309.1	DUF987 domain-containing protein	-
F1745_RS20930 (4223141)	4223141..4223515	+	375	WP_096848639.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F1745_RS20935 (4223562)	4223562..4223939	+	378	WP_096848640.1	TA system toxin CbtA family protein	Toxin
F1745_RS20940 (4223936)	4223936..4224424	+	489	WP_096848642.1	DUF5983 family protein	-
F1745_RS20945 (4224436)	4224436..4224633	+	198	WP_000772034.1	DUF957 domain-containing protein	-
F1745_RS20950 (4224718)	4224718..4225560	+	843	WP_096848643.1	DUF4942 domain-containing protein	-
F1745_RS20955 (4225798)	4225798..4225959	+	162	Protein_4106	virulence RhuM family protein	-
F1745_RS20960 (4226356)	4226356..4227540	+	1185	WP_001172876.1	sugar efflux transporter	-
F1745_RS20965 (4227651)	4227651..4228574	+	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14031.04 Da
• Isoelectric Point: 8.2904

>T139321 WP_096848640.1 NZ_CP043825:4223562-4223939 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQALLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T139321 NZ_CP058638:5354173-5354526 [Rhodococcus sp. ZPP]
GTGTTCAACGTAGAACTCTTGAAGGAGGTTGAAGAATGGTTCATCGAACTTGCTTCGACCGACCCAGAGGCTGCCGAGCA
TGTTGCTGGTGCGATTGACCTTCTCGAGGCTACCGGTCCAACCTTAGGGCGCCCAGTGGTCGACAAGGTGAAAGCCTCTC
GCTTTCACAGCATGAAGGAGTTGCGACCGCCGGGTACTCACATACGAATCTTGTTCATTTTTGATCCACACCGCGATGCG
GTACTGCTAGTCGGCGGCGATAAGACTGGTCAATGGCGGGCGTGGTATGCCGAAAACATCCCGGTGGCGGACGACCGGTA
TCAGAAGCGGCTAGACGGAGATTACGACGACTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13719.43 Da
• Isoelectric Point: 5.8640

>AT139321 WP_096848639.1 NZ_CP043825:4223141-4223515 [Escherichia coli]
VSDTHPGTTHPDNNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRVGIRGQFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLVPATTS

Nucleotide

• Length: 375 bp

>AT139321 NZ_CP058638:5354541-5354858 [Rhodococcus sp. ZPP]
GTGGCACGGAATTGGCGTGAGGTTCGCGCGGAGGCGGTCGACAACGGATTGATCGATCCGCAGCGCGCGGACAACGCACG
TAAGGAGATGCATGAGATGGTGCGCGCCTACCGCCTGGCAGACATCCGTAAGGCCCAAGGGCCGCGGCAGGCGGATGTTG
CTAAGGCGCTAGGTGTCTCACAAGCCCGAGTGTCGAAGCTCGAACGCGGCGACTTGCAACACACGGAGTTGGGCACCTTG
GAATCGTATGTGGCTGCGCTCGGCGGACGCCTACGCGTGGTGGCGGACTTCGGAGACGACTCGATCACACTTCAGTAG