Detailed information of TA system TA139320

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4161541..4162037
• Replicon: chromosome
• Accession: NZ_CP043825
• Organism: Escherichia coli strain RM8082

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: F1745_RS20670
• Protein ID: WP_001173343.1
• Coordinates: 4161864..4162037 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: F1745_RS20665
• Protein ID: WP_096848711.1
• Coordinates: 4161541..4161837 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1745_RS20635 (4157666)	4157666..4157859	+	194	Protein_4042	transposase	-
F1745_RS20640 (4157945)	4157945..4158274	+	330	WP_096848718.1	hypothetical protein	-
F1745_RS20645 (4158261)	4158261..4158653	+	393	WP_096848717.1	IS66 family insertion sequence element accessory protein TnpB	-
F1745_RS20650 (4158660)	4158660..4160261	+	1602	WP_096848715.1	IS66 family transposase	-
F1745_RS20655 (4160776)	4160776..4160961	-	186	Protein_4046	transposase domain-containing protein	-
F1745_RS20660 (4161213)	4161213..4161497	+	285	WP_096848713.1	hypothetical protein	-
F1745_RS20665 (4161541)	4161541..4161837	+	297	WP_096848711.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
F1745_RS20670 (4161864)	4161864..4162037	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
F1745_RS20675 (4162156)	4162156..4163673	-	1518	WP_238013691.1	lysine--tRNA ligase	-
F1745_RS20680 (4163988)	4163988..4166135	-	2148	WP_238013694.1	lysine decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4123569..4177956	54387

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (6-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T139320 WP_001173343.1 NZ_CP043825:4161864-4162037 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T139320 NZ_CP058638:3634740-3635150 [Rhodococcus sp. ZPP]
ATGAGTGGCTCCACGTGGCTCATCGACAAGTCCGCGATGGTGCGCCTGGGCACCAGCCCGGACGCCGAACAGTGGGCCAA
GCGAATCCAGCGCGGTCTCGTCCACATCTGCACCGTGACGCTGCTCGAGGTCGGCTGCTCGGCACGTACCGCCCACGACC
TGTCGCGGGGACGACGCACCCCACCGCTATCGGCCATGCCCCTGGAGTATTCGACACCCGCGGTCGAAGACCGCGCCCTT
GATGTGCAACGGCAACTGGCTGAACGTGGCCAACATCGCGCACCATCGATCCCGGATCTGCTCATCGCGGCGACCGCGGA
AATCAACAACCGAGTCGTTCTCCATCTGGACAAAGACTTCGATCTGATCGCGGCGATAACCAGCCAACCACTCGAACGAC
TGGTGGCATAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11498.12 Da
• Isoelectric Point: 4.2404

>AT139320 WP_096848711.1 NZ_CP043825:4161541-4161837 [Escherichia coli]
MKCKNRFNTYVVSFDYPSSYSSVFLMLRSLMYDMNFSSIVADEYGIPRQLNENSLAITTSLSASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT139320 NZ_CP058638:3634522-3634743 [Rhodococcus sp. ZPP]
ATGCCCGATATTTTGATCCGCAACTTTCCCGCCGAGGATCTGGCGCTGCTCGACGAGCACGCTGCCCGTCTCGGTATCTC
GCGAACTGAATTCCTGCGCCGTCAGCTGCAGCAGGAGGCACGTCGCACCGCCACCTCGGTGTCGACCGCCGACCTGATGC
GGTTCTCGCAGGACTTCCCCGATTTGAACGACAATGAGGTCATGCGCGGCGCCTGGTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U9QI41