Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3895875..3896461 Replicon chromosome
Accession NZ_CP043825
Organism Escherichia coli strain RM8082

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag F1745_RS19500 Protein ID WP_042014162.1
Coordinates 3896231..3896461 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag F1745_RS19495 Protein ID WP_000593555.1
Coordinates 3895875..3896234 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1745_RS19470 (3892007) 3892007..3892951 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
F1745_RS19475 (3892948) 3892948..3893781 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
F1745_RS19480 (3893781) 3893781..3894545 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
F1745_RS19485 (3894542) 3894542..3895348 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
F1745_RS19490 (3895354) 3895354..3895755 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
F1745_RS19495 (3895875) 3895875..3896234 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
F1745_RS19500 (3896231) 3896231..3896461 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
F1745_RS19505 (3896565) 3896565..3897689 - 1125 WP_001314210.1 ABC transporter permease -
F1745_RS19510 (3897689) 3897689..3900424 - 2736 WP_000149158.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T139315 WP_042014162.1 NZ_CP043825:c3896461-3896231 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T139315 NZ_CP043825:c3896461-3896231 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT139315 WP_000593555.1 NZ_CP043825:c3896234-3895875 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT139315 NZ_CP058637:3248131-3248433 [Enterobacter roggenkampii]
ATGAAGGAGATTGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAACTTAAAAGATCGACGAGCGAAGACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGCTTAGCGGGCGACGTTAAACCCGTGGGAGAAGGTATAAGTGAGCTAAGGATCC
ACTTTGGTCCGGGCTACAGGATCTATTTTAAAGACCAGGGCAATTACATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTGATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References