Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3208775..3209429 Replicon chromosome
Accession NZ_CP043825
Organism Escherichia coli strain RM8082

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag F1745_RS15985 Protein ID WP_000244772.1
Coordinates 3208775..3209182 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag F1745_RS15990 Protein ID WP_000354046.1
Coordinates 3209163..3209429 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1745_RS15965 (3204732) 3204732..3206465 - 1734 WP_238013613.1 single-stranded-DNA-specific exonuclease RecJ -
F1745_RS15970 (3206471) 3206471..3207181 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
F1745_RS15975 (3207206) 3207206..3208102 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
F1745_RS15980 (3208214) 3208214..3208735 + 522 WP_001055874.1 flavodoxin FldB -
F1745_RS15985 (3208775) 3208775..3209182 - 408 WP_000244772.1 protein YgfX Toxin
F1745_RS15990 (3209163) 3209163..3209429 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
F1745_RS15995 (3209672) 3209672..3210652 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
F1745_RS16000 (3210848) 3210848..3211507 - 660 WP_000250274.1 hemolysin III family protein -
F1745_RS16005 (3211671) 3211671..3211982 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
F1745_RS16010 (3212027) 3212027..3213460 + 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
F1745_RS16015 (3213517) 3213517..3214260 - 744 WP_000951961.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T139313 WP_000244772.1 NZ_CP043825:c3209182-3208775 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T139313 NZ_CP058637:2434498-2434716 [Enterobacter roggenkampii]
ATGTCTGATAAACCACTCACTAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTCGAACGCGT
GATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCCGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAGC
TGACCATGAATAAGTTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT139313 WP_000354046.1 NZ_CP043825:c3209429-3209163 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT139313 NZ_CP058637:2434096-2434470 [Enterobacter roggenkampii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAACCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTTGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References