Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3067320..3067903 Replicon chromosome
Accession NZ_CP043825
Organism Escherichia coli strain RM8082

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag F1745_RS15345 Protein ID WP_000254745.1
Coordinates 3067320..3067655 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2J1DJJ7
Locus tag F1745_RS15350 Protein ID WP_000581939.1
Coordinates 3067655..3067903 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1745_RS15330 (3063207) 3063207..3064505 - 1299 WP_000036723.1 phosphopyruvate hydratase -
F1745_RS15335 (3064593) 3064593..3066230 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
F1745_RS15340 (3066458) 3066458..3067249 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
F1745_RS15345 (3067320) 3067320..3067655 - 336 WP_000254745.1 endoribonuclease MazF Toxin
F1745_RS15350 (3067655) 3067655..3067903 - 249 WP_000581939.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
F1745_RS15355 (3067981) 3067981..3070215 - 2235 WP_000226815.1 GTP pyrophosphokinase -
F1745_RS15360 (3070263) 3070263..3071564 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3061824..3063152 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

Antitoxin PemI-like

(2-82)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T139312 WP_000254745.1 NZ_CP043825:c3067655-3067320 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T139312 NZ_CP058637:692840-692943 [Enterobacter roggenkampii]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9369.76 Da        Isoelectric Point: 4.4791

>AT139312 WP_000581939.1 NZ_CP043825:c3067903-3067655 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNEITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT139312 NZ_CP058637:c692950-692805 [Enterobacter roggenkampii]
AACAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
AlphaFold DB A0A2J1DJJ7

References