Detailed information of TA system TA139299

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1623524..1624162
• Replicon: chromosome
• Accession: NZ_CP043825
• Organism: Escherichia coli strain RM8082

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: F1745_RS08235
• Protein ID: WP_000813794.1
• Coordinates: 1623524..1623700 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: F1745_RS08240
• Protein ID: WP_001270286.1
• Coordinates: 1623746..1624162 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1745_RS08215 (1619143)	1619143..1620357	-	1215	WP_071609973.1	BenE family transporter YdcO	-
F1745_RS08220 (1620410)	1620410..1620946	+	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
F1745_RS08225 (1621019)	1621019..1622980	+	1962	WP_001442195.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
F1745_RS08230 (1623072)	1623072..1623302	-	231	WP_000494244.1	YncJ family protein	-
F1745_RS08235 (1623524)	1623524..1623700	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
F1745_RS08240 (1623746)	1623746..1624162	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
F1745_RS08245 (1624241)	1624241..1625647	+	1407	WP_000760663.1	PLP-dependent aminotransferase family protein	-
F1745_RS08250 (1625892)	1625892..1627037	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
F1745_RS08255 (1627055)	1627055..1628068	+	1014	WP_001542893.1	ABC transporter ATP-binding protein	-
F1745_RS08260 (1628069)	1628069..1629010	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1617955..1619103	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T139299 WP_000813794.1 NZ_CP043825:1623524-1623700 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T139299 NZ_CP043825:1623524-1623700 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT139299 WP_001270286.1 NZ_CP043825:1623746-1624162 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT139299 NZ_CP043825:1623746-1624162 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB