Detailed information of TA system TA139296

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 56159..56706
• Replicon: plasmid p1RM10740
• Accession: NZ_CP043824
• Organism: Escherichia coli strain RM10740

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G7QEJ9
• Locus tag: F1748_RS26240
• Protein ID: WP_001384452.1
• Coordinates: 56159..56437 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: K4JYR0
• Locus tag: F1748_RS26245
• Protein ID: WP_000079928.1
• Coordinates: 56437..56706 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1748_RS26220 (52383)	52383..53180	-	798	WP_000544830.1	IS21-like element IS21 family helper ATPase IstB	-
F1748_RS26225 (53180)	53180..54352	-	1173	WP_089516343.1	IS21-like element IS21 family transposase	-
F1748_RS26230 (54454)	54454..55968	+	1515	Protein_78	Tn3-like element ISEc63 family transposase	-
F1748_RS26235 (56001)	56001..56123	-	123	Protein_79	3'-5' exonuclease	-
F1748_RS26240 (56159)	56159..56437	-	279	WP_001384452.1	type II toxin-antitoxin system toxin YacB	Toxin
F1748_RS26245 (56437)	56437..56706	-	270	WP_000079928.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
F1748_RS26250 (56820)	56820..57601	-	782	Protein_82	IS21-like element IS100kyp family helper ATPase IstB	-
F1748_RS26255 (57598)	57598..57987	-	390	Protein_83	IS21 family transposase	-
F1748_RS26260 (58076)	58076..58477	+	402	WP_000124097.1	hypothetical protein	-
F1748_RS26265 (58440)	58440..59627	+	1188	WP_000937612.1	IS91-like element IS91 family transposase	-
F1748_RS26270 (59808)	59808..60071	-	264	WP_236411453.1	DNA distortion polypeptide 3	-
F1748_RS26275 (60208)	60208..60654	-	447	WP_000057426.1	hypothetical protein	-
F1748_RS26280 (60694)	60694..61530	-	837	WP_001050931.1	RepB family plasmid replication initiator protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul3 / tet(A)	paa / hlyD / hlyB / hlyA / hlyC	1..114358	114358
	-	inside	IScluster/Tn	sul3	-	41510..59627	18117

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10762.38 Da
• Isoelectric Point: 8.4615

>T139296 WP_001384452.1 NZ_CP043824:c56437-56159 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T139296 NZ_CP058618:3869521-3869928 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10175.50 Da
• Isoelectric Point: 7.3210

>AT139296 WP_000079928.1 NZ_CP043824:c56706-56437 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT139296 NZ_CP058618:3869274-3869540 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829CL36

Antitoxin

• AlphaFold DB: A0A5W2J4D0