Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 72482..73285 Replicon plasmid p2RM10740
Accession NZ_CP043823
Organism Escherichia coli strain RM10740

Toxin (Protein)


Gene name KacT Uniprot ID A0A0K3X5E3
Locus tag F1748_RS25710 Protein ID WP_000348884.1
Coordinates 72482..73012 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID G3CAI7
Locus tag F1748_RS25715 Protein ID WP_001275013.1
Coordinates 73016..73285 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1748_RS25680 (67606) 67606..67911 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
F1748_RS25685 (67913) 67913..68131 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA -
F1748_RS25690 (68844) 68844..71465 + 2622 WP_023063774.1 ABC transporter -
F1748_RS25695 (71664) 71664..71894 + 231 WP_001261274.1 type II toxin-antitoxin system VapB family antitoxin -
F1748_RS25700 (71891) 71891..72207 + 317 Protein_74 type II toxin-antitoxin system VapC family toxin -
F1748_RS25705 (72267) 72267..72481 - 215 Protein_75 transposase -
F1748_RS25710 (72482) 72482..73012 - 531 WP_000348884.1 GNAT family N-acetyltransferase Toxin
F1748_RS25715 (73016) 73016..73285 - 270 WP_001275013.1 DUF1778 domain-containing protein Antitoxin
F1748_RS25720 (74185) 74185..75162 + 978 WP_089516180.1 RepB family plasmid replication initiator protein -
F1748_RS25725 (75447) 75447..76187 - 741 WP_001066942.1 tyrosine-type recombinase/integrase -
F1748_RS25730 (76308) 76308..76496 - 189 WP_238005213.1 serine/threonine protein kinase -
F1748_RS25740 (77538) 77538..78044 - 507 WP_024185287.1 thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..100386 100386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20320.22 Da        Isoelectric Point: 6.7486

>T139292 WP_000348884.1 NZ_CP043823:c73012-72482 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEVLFEVNDE

Download         Length: 531 bp

>T139292 NZ_CP058618:c2499724-2499551 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10229.63 Da        Isoelectric Point: 6.4702

>AT139292 WP_001275013.1 NZ_CP043823:c73285-73016 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Download         Length: 270 bp

>AT139292 NZ_CP058618:c2500048-2499752 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K3X5E3

Antitoxin

Source ID Structure
AlphaFold DB G3CAI7

References