Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 67606..68131 Replicon plasmid p2RM10740
Accession NZ_CP043823
Organism Escherichia coli strain RM10740

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag F1748_RS25680 Protein ID WP_001159871.1
Coordinates 67606..67911 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag F1748_RS25685 Protein ID WP_000813639.1
Coordinates 67913..68131 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1748_RS25660 (63665) 63665..64039 - 375 WP_000752649.1 hypothetical protein -
F1748_RS25665 (64039) 64039..64671 - 633 WP_000312330.1 ParA family protein -
F1748_RS25670 (65519) 65519..66274 - 756 WP_000807692.1 replication initiation protein RepE -
F1748_RS25675 (66799) 66799..67605 - 807 WP_089516183.1 site-specific integrase -
F1748_RS25680 (67606) 67606..67911 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
F1748_RS25685 (67913) 67913..68131 - 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
F1748_RS25690 (68844) 68844..71465 + 2622 WP_023063774.1 ABC transporter -
F1748_RS25695 (71664) 71664..71894 + 231 WP_001261274.1 type II toxin-antitoxin system VapB family antitoxin -
F1748_RS25700 (71891) 71891..72207 + 317 Protein_74 type II toxin-antitoxin system VapC family toxin -
F1748_RS25705 (72267) 72267..72481 - 215 Protein_75 transposase -
F1748_RS25710 (72482) 72482..73012 - 531 WP_000348884.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..100386 100386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T139291 WP_001159871.1 NZ_CP043823:c67911-67606 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T139291 NZ_CP058618:2205824-2206033 [Escherichia coli]
ATGCTGAACACATGTAGAGTGCCTCTTACTGACCGTAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT139291 WP_000813639.1 NZ_CP043823:c68131-67913 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT139291 NZ_CP058618:c2205832-2205775 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References