Detailed information of TA system TA139285

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5007451..5009134
• Replicon: chromosome
• Accession: NZ_CP043821
• Organism: Escherichia coli strain RM10740

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: F1748_RS24595
• Protein ID: WP_000361918.1
• Coordinates: 5007451..5008368 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: I2UPX5
• Locus tag: F1748_RS24600
• Protein ID: WP_000157137.1
• Coordinates: 5008361..5009134 (-)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1748_RS24575 (5002469)	5002469..5004802	+	2334	WP_001593805.1	primase-like DNA-binding domain-containing protein	-
F1748_RS24580 (5005008)	5005008..5005343	+	336	WP_077778672.1	integrase	-
F1748_RS24585 (5005561)	5005561..5006202	+	642	Protein_4791	integrase arm-type DNA-binding domain-containing protein	-
F1748_RS24590 (5006275)	5006275..5006937	+	663	WP_000357928.1	hypothetical protein	-
F1748_RS24595 (5007451)	5007451..5008368	-	918	WP_000361918.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
F1748_RS24600 (5008361)	5008361..5009134	-	774	WP_000157137.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
F1748_RS24605 (5009218)	5009218..5009538	+	321	Protein_4795	IS3 family transposase	-
F1748_RS24610 (5009714)	5009714..5011063	-	1350	WP_000766444.1	phospholipase D-like domain-containing protein	-
F1748_RS24615 (5011182)	5011182..5013266	-	2085	WP_032192226.1	protease Lon-related BREX system protein BrxL	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4989468..5015182	25714

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34962.10 Da
• Isoelectric Point: 4.9206

>T139285 WP_000361918.1 NZ_CP043821:c5008368-5007451 [Escherichia coli]
MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPRLSVDIDLAWIPLESREVALPNVREALTRIAAELKAQ
TGIRAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMTFEEVSLEELKEVPERMLSTLKT
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGQMSEEKRAQSLEKLEKVMTEWLR

Nucleotide

• Length: 918 bp

>T139285 NZ_CP058615:2082288-2082476 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29333.76 Da
• Isoelectric Point: 9.9889

>AT139285 WP_000157137.1 NZ_CP043821:c5009134-5008361 [Escherichia coli]
MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRIDTSKINLGSGNRQIVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

>AT139285 NZ_CP058615:2081999-2082259 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H0N3W2