Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3496701..3497355 Replicon chromosome
Accession NZ_CP043821
Organism Escherichia coli strain RM10740

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag F1748_RS17345 Protein ID WP_000244772.1
Coordinates 3496701..3497108 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag F1748_RS17350 Protein ID WP_000354046.1
Coordinates 3497089..3497355 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1748_RS17325 (3492658) 3492658..3494391 - 1734 WP_238005023.1 single-stranded-DNA-specific exonuclease RecJ -
F1748_RS17330 (3494397) 3494397..3495107 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
F1748_RS17335 (3495132) 3495132..3496028 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
F1748_RS17340 (3496140) 3496140..3496661 + 522 WP_001055874.1 flavodoxin FldB -
F1748_RS17345 (3496701) 3496701..3497108 - 408 WP_000244772.1 protein YgfX Toxin
F1748_RS17350 (3497089) 3497089..3497355 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
F1748_RS17355 (3497598) 3497598..3498578 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
F1748_RS17360 (3498655) 3498655..3499314 - 660 WP_000250274.1 hemolysin III family protein -
F1748_RS17365 (3499478) 3499478..3499789 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
F1748_RS17370 (3499834) 3499834..3501267 + 1434 WP_238005024.1 6-phospho-beta-glucosidase BglA -
F1748_RS17375 (3501324) 3501324..3502067 - 744 WP_001436959.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T139278 WP_000244772.1 NZ_CP043821:c3497108-3496701 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T139278 NZ_CP058615:466674-466940 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT139278 WP_000354046.1 NZ_CP043821:c3497355-3497089 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT139278 NZ_CP058615:466423-466674 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References