Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1705105..1705743 Replicon chromosome
Accession NZ_CP043821
Organism Escherichia coli strain RM10740

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag F1748_RS08490 Protein ID WP_000813794.1
Coordinates 1705105..1705281 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag F1748_RS08495 Protein ID WP_001270286.1
Coordinates 1705327..1705743 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1748_RS08470 (1700724) 1700724..1701938 - 1215 WP_001307190.1 BenE family transporter YdcO -
F1748_RS08475 (1701991) 1701991..1702527 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
F1748_RS08480 (1702600) 1702600..1704561 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
F1748_RS08485 (1704653) 1704653..1704883 - 231 WP_000494244.1 YncJ family protein -
F1748_RS08490 (1705105) 1705105..1705281 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
F1748_RS08495 (1705327) 1705327..1705743 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
F1748_RS08500 (1705822) 1705822..1707228 + 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
F1748_RS08505 (1707473) 1707473..1708618 + 1146 WP_089516161.1 ABC transporter substrate-binding protein -
F1748_RS08510 (1708636) 1708636..1709649 + 1014 WP_000220411.1 ABC transporter ATP-binding protein -
F1748_RS08515 (1709650) 1709650..1710591 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T139264 WP_000813794.1 NZ_CP043821:1705105-1705281 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T139264 NZ_CP058596:c4987687-4987376 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT139264 WP_001270286.1 NZ_CP043821:1705327-1705743 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT139264 NZ_CP058596:c4987375-4987085 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References