Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 569515..570133 Replicon chromosome
Accession NZ_CP043821
Organism Escherichia coli strain RM10740

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag F1748_RS02700 Protein ID WP_001291435.1
Coordinates 569515..569733 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag F1748_RS02705 Protein ID WP_000344800.1
Coordinates 569759..570133 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F1748_RS02665 (564816) 564816..565388 + 573 WP_000779826.1 YbaY family lipoprotein -
F1748_RS02670 (565419) 565419..565730 - 312 WP_000409911.1 MGMT family protein -
F1748_RS02680 (566109) 566109..566462 + 354 WP_000878141.1 DUF1428 family protein -
F1748_RS02685 (566504) 566504..568054 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
F1748_RS02690 (568218) 568218..568688 - 471 WP_000136192.1 YlaC family protein -
F1748_RS02695 (568804) 568804..569343 - 540 WP_089515649.1 maltose O-acetyltransferase -
F1748_RS02700 (569515) 569515..569733 - 219 WP_001291435.1 HHA domain-containing protein Toxin
F1748_RS02705 (569759) 569759..570133 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
F1748_RS02710 (570679) 570679..573828 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
F1748_RS02715 (573851) 573851..575044 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T139259 WP_001291435.1 NZ_CP043821:c569733-569515 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T139259 NZ_CP058596:4333105-4333314 [Escherichia coli]
ATGCAGAACACATGTAGAGTGCCTCTTACTGACCGAAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT139259 WP_000344800.1 NZ_CP043821:c570133-569759 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT139259 NZ_CP058596:c4333113-4333056 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References