Detailed information of TA system TA139258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 535230..536067
• Replicon: chromosome
• Accession: NZ_CP043821
• Organism: Escherichia coli strain RM10740

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: F1748_RS02530
• Protein ID: WP_000227784.1
• Coordinates: 535230..535772 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: F1748_RS02535
• Protein ID: WP_001297137.1
• Coordinates: 535756..536067 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1748_RS02510 (530769)	530769..531680	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
F1748_RS02515 (531848)	531848..532339	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
F1748_RS02520 (532467)	532467..533831	-	1365	WP_001000978.1	MFS transporter	-
F1748_RS02525 (534239)	534239..535174	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
F1748_RS02530 (535230)	535230..535772	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
F1748_RS02535 (535756)	535756..536067	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
F1748_RS02540 (536252)	536252..537142	-	891	WP_089515655.1	heme o synthase	-
F1748_RS02545 (537154)	537154..537483	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
F1748_RS02550 (537483)	537483..538097	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
F1748_RS02555 (538087)	538087..540078	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
F1748_RS02560 (540100)	540100..541047	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T139258 WP_000227784.1 NZ_CP043821:c535772-535230 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T139258 NZ_CP058596:c4296244-4295930 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT139258 WP_001297137.1 NZ_CP043821:c536067-535756 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT139258 NZ_CP058596:c4296480-4296247 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3