Detailed information of TA system TA139251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta(toxin)
• Location: 7570..9221
• Replicon: plasmid p1
• Accession: NZ_CP043817
• Organism: Neisseria gonorrhoeae strain NG251

Toxin (Protein)

• Gene name: ng_zeta1
• Uniprot ID: -
• Locus tag: F0T11_RS11655
• Protein ID: WP_033911112.1
• Coordinates: 8016..9221 (-)
• Length: 402 a.a.

Antitoxin (Protein)

• Gene name: ng_epsilon1
• Uniprot ID: D5K9G8
• Locus tag: F0T11_RS11650
• Protein ID: WP_003701903.1
• Coordinates: 7570..8001 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0T11_RS11620	2679..3455	+	777	WP_003701891.1	P-type conjugative transfer protein TrbJ	-
F0T11_RS11625	3669..5297	+	1629	WP_047920931.1	P-type conjugative transfer protein TrbL	-
F0T11_RS11630	5284..5862	+	579	WP_033911116.1	TrbM protein	-
F0T11_RS11635	5862..6461	+	600	WP_003701898.1	TrbN protein	-
F0T11_RS11640	6564..6854	+	291	WP_003701899.1	virulence factor	-
F0T11_RS11645	6964..7518	-	555	WP_003701901.1	recombinase family protein	-
F0T11_RS11650	7570..8001	-	432	WP_003701903.1	hypothetical protein	Antitoxin
F0T11_RS11655	8016..9221	-	1206	WP_033911112.1	zeta toxin family protein	Toxin
F0T11_RS11660	9228..9413	-	186	WP_014580058.1	hypothetical protein	-
F0T11_RS11665	9447..10655	-	1209	WP_014580057.1	zeta toxin family protein	-
F0T11_RS11670	10652..10900	-	249	WP_014580056.1	hypothetical protein	-
F0T11_RS11675	11545..11661	+	117	WP_001791010.1	tetracycline resistance determinant leader peptide	-
F0T11_RS11680	11677..13596	+	1920	WP_025456273.1	tetracycline resistance ribosomal protection protein Tet(M)	-
F0T11_RS11685	13673..14161	+	489	WP_003701915.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(M)	-	1..41356	41356

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• KfrB domain (284-343)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 402 a.a.
• Molecular weight: 44747.59 Da
• Isoelectric Point: 7.2930

>T139251 WP_033911112.1 NZ_CP043817:c9221-8016 [Neisseria gonorrhoeae]
MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQHADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHN
SAAVLSSTQFDEVLKRDEPQKKEVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
VSMRAERASDNTYKRFNEYGREASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQDRNSEFIDKFDDVIKMLSLGSQE
QILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESVFAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGI
YLLGQAKKAETGQTYSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLEYSAKKSIS
R

Nucleotide

• Length: 1206 bp

>T139251 NZ_CP043817:c9221-8016 [Neisseria gonorrhoeae]
ATGGTAAAACTGTCTAGCGATATTAATCTGAGAGATTTTGGCAATAACGAATATTTGTCTTCCGTTCAGGATGAGGCCAT
CCGTTTTGCGACCGAACAAACAGACGAGATATTGTCTTTATATAGCCAACATGCAGATACAGAAGGCGGTAGATATGTTT
GTGCTGATACGTTTAAAGAGTTGTTTCCAGCTTTTGAGAACAAAGAAGACAGGGCAACGGTTAATAATGCCATCCACAAT
TCAGCAGCAGTTTTATCTTCTACCCAGTTTGACGAGGTACTGAAACGCGACGAGCCACAGAAGAAAGAAGTTATTTTCGT
TACGGGAATTCCGGGTTCGGGAAAAACTTCGACAGTAAAAAACATGATGATGCAGGATACCACCAAGCTGCTTTTTGAAG
GACAGCTTGCACGGCCGCAGTCTGCTTTTCGCAAGATTGAGCAGTGTCTGGAGCGTAATCTTGAAGTTACTATCGTAGCC
GTCAGCATGAGGGCTGAAAGGGCTTCCGACAATACTTACAAACGGTTTAACGAATACGGCAGGGAAGCCAGTATAGGTAT
TATGGCTGATATTCAGGCCAATCTTCCTGACGGACTCAAGCAAATCAGGGATAAGTTTGGTGATGCGGTAAAAATTGTCG
GTATCAACCAAGACAGGAACAGTGAATTTATCGACAAGTTCGATGATGTCATCAAAATGCTTTCCTTGGGGTCGCAAGAG
CAAATATTGGGCAGGTTGGCCGAGAAAATCCAGAGCGACTTCGACTCAGGAAAAATCAGCCGGGAATGCTTCAATCAGGC
CAAGGGCAGTATGGATCTTGAAAGTGTTTTCGCCAAAAAAGAGTATTCCCAGCAAAGGGTAGTTACTAACTCCAAGGGTG
TTACCCTAGAAACAAAGTCAGCAAACGAGTTGTGGTCTAAGGTGGAACAAATTCCTGTAACAGGCATGAAAGCCGGTATC
TATTTACTAGGTCAAGCTAAAAAAGCAGAGACTGGTCAAACTTATTCCGGAGAAATCATTTACAAGGATGCCGCTGCGGT
ATTTCAAAAAACCAAAAACGGGCTTGTCAGGCACAATGCAACACATAACGAAGAAAGACTGGCCAAATTGGTTGAGATAG
GGCAGAACGTTTCTATCGGAAGCAATAAGGGCAAATTGATTGTCAAGTCTTTGGAGTATTCCGCCAAAAAATCCATCAGC
AGATAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15927.05 Da
• Isoelectric Point: 4.4211

>AT139251 WP_003701903.1 NZ_CP043817:c8001-7570 [Neisseria gonorrhoeae]
MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLADILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVR
LSPFLNGCPHDLSLGAESLGKGESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ

Nucleotide

• Length: 432 bp

>AT139251 NZ_CP058596:c2261226-2260958 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Antitoxin

• AlphaFold DB: D5K9G8