Detailed information of TA system TA139248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 1201..1664
• Replicon: plasmid p3
• Accession: NZ_CP043815
• Organism: Neisseria gonorrhoeae strain NG290

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: Q51003
• Locus tag: F0T12_RS11900
• Protein ID: WP_003690225.1
• Coordinates: 1386..1664 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: Q51002
• Locus tag: F0T12_RS11895
• Protein ID: WP_003690223.1
• Coordinates: 1201..1386 (+)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0T12_RS11885	714..956	+	243	WP_003690220.1	HTH domain-containing protein	-
F0T12_RS11890	953..1198	+	246	WP_003690221.1	hypothetical protein	-
F0T12_RS11895	1201..1386	+	186	WP_003690223.1	DUF5397 family protein	Antitoxin
F0T12_RS11900	1386..1664	+	279	WP_003690225.1	endoribonuclease VapD	Toxin
F0T12_RS11905	1642..1911	-	270	WP_003692166.1	hypothetical protein	-
F0T12_RS11910	1943..2584	-	642	WP_003690229.1	Cryptic plasmid protein B	-
F0T12_RS11915	2668..3297	-	630	WP_003696452.1	relaxase/mobilization nuclease domain-containing protein	-
F0T12_RS11920	3282..3473	-	192	WP_003690218.1	plasmid mobilization relaxosome protein MobC	-
F0T12_RS11925	3741..3965	+	225	WP_025455920.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..4207	4207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (2-60)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10473.89 Da
• Isoelectric Point: 6.9726

>T139248 WP_003690225.1 NZ_CP043815:1386-1664 [Neisseria gonorrhoeae]
MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSLYTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRI
EQWSDFTSLVKS

Nucleotide

• Length: 279 bp

>T139248 NZ_CP058596:1960605-1960856 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 6717.54 Da
• Isoelectric Point: 4.2641

>AT139248 WP_003690223.1 NZ_CP043815:1201-1386 [Neisseria gonorrhoeae]
MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESGEHTQYKLSHIVQDPKAA

Nucleotide

• Length: 186 bp

>AT139248 NZ_CP058596:1960853-1961107 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A7H5FJI5

Antitoxin

• AlphaFold DB: Q51002