Detailed information of TA system TA139247

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta(toxin)
• Location: 36818..38469
• Replicon: plasmid p1
• Accession: NZ_CP043813
• Organism: Neisseria gonorrhoeae strain NG290

Toxin (Protein)

• Gene name: ng_zeta1
• Uniprot ID: -
• Locus tag: F0T12_RS11820
• Protein ID: WP_033911112.1
• Coordinates: 37264..38469 (-)
• Length: 402 a.a.

Antitoxin (Protein)

• Gene name: ng_epsilon1
• Uniprot ID: D5K9G8
• Locus tag: F0T12_RS11815
• Protein ID: WP_003701903.1
• Coordinates: 36818..37249 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0T12_RS11785	31927..32703	+	777	WP_003701891.1	P-type conjugative transfer protein TrbJ	-
F0T12_RS11790	32917..34545	+	1629	WP_047920931.1	P-type conjugative transfer protein TrbL	-
F0T12_RS11795	34532..35110	+	579	WP_033911116.1	TrbM protein	-
F0T12_RS11800	35110..35709	+	600	WP_003701898.1	TrbN protein	-
F0T12_RS11805	35812..36102	+	291	WP_003701899.1	virulence factor	-
F0T12_RS11810	36212..36766	-	555	WP_003701901.1	recombinase family protein	-
F0T12_RS11815	36818..37249	-	432	WP_003701903.1	hypothetical protein	Antitoxin
F0T12_RS11820	37264..38469	-	1206	WP_033911112.1	zeta toxin family protein	Toxin
F0T12_RS11825	38476..38661	-	186	WP_014580058.1	hypothetical protein	-
F0T12_RS11830	38695..39903	-	1209	WP_014580057.1	zeta toxin family protein	-
F0T12_RS11835	39900..40148	-	249	WP_014580056.1	hypothetical protein	-
F0T12_RS11840	40793..40909	+	117	WP_001791010.1	tetracycline resistance determinant leader peptide	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..41357	41357

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• KfrB domain (284-343)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 402 a.a.
• Molecular weight: 44747.59 Da
• Isoelectric Point: 7.2930

>T139247 WP_033911112.1 NZ_CP043813:c38469-37264 [Neisseria gonorrhoeae]
MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQHADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHN
SAAVLSSTQFDEVLKRDEPQKKEVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
VSMRAERASDNTYKRFNEYGREASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQDRNSEFIDKFDDVIKMLSLGSQE
QILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESVFAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGI
YLLGQAKKAETGQTYSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLEYSAKKSIS
R

Nucleotide

• Length: 1206 bp

>T139247 NZ_CP058596:1265940-1266251 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15927.05 Da
• Isoelectric Point: 4.4211

>AT139247 WP_003701903.1 NZ_CP043813:c37249-36818 [Neisseria gonorrhoeae]
MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLADILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVR
LSPFLNGCPHDLSLGAESLGKGESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ

Nucleotide

• Length: 432 bp

>AT139247 NZ_CP058596:1266248-1266667 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Antitoxin

• AlphaFold DB: D5K9G8