Detailed information of TA system TA139233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 259219..259748
• Replicon: chromosome
• Accession: NZ_CP043808
• Organism: Serinicoccus marinus DSM 15273

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1B1NCA9
• Locus tag: FU792_RS01275
• Protein ID: WP_022923225.1
• Coordinates: 259219..259545 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: FU792_RS01280
• Protein ID: WP_066638586.1
• Coordinates: 259542..259748 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FU792_RS01235	254484..254879	+	396	WP_022923232.1	hypothetical protein	-
FU792_RS01240	254907..255482	-	576	WP_149814478.1	hypothetical protein	-
FU792_RS01245	255699..256412	+	714	WP_028130711.1	class I SAM-dependent methyltransferase	-
FU792_RS01250	256442..256945	-	504	WP_022923229.1	DinB family protein	-
FU792_RS01255	257113..257583	+	471	WP_168714885.1	iron-sulfur cluster assembly accessory protein	-
FU792_RS01260	257684..258256	+	573	WP_022923228.1	class I SAM-dependent methyltransferase	-
FU792_RS01265	258437..258841	+	405	WP_022923227.1	hypothetical protein	-
FU792_RS01270	258834..259037	+	204	WP_022923226.1	helix-turn-helix transcriptional regulator	-
FU792_RS01275	259219..259545	-	327	WP_022923225.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FU792_RS01280	259542..259748	-	207	WP_066638586.1	DUF3018 family protein	Antitoxin
FU792_RS01285	259934..260986	-	1053	WP_022926335.1	hypothetical protein	-
FU792_RS01290	261041..262338	+	1298	Protein_256	IS3 family transposase	-
FU792_RS01295	262505..262765	-	261	WP_149814479.1	hypothetical protein	-
FU792_RS01300	262879..263205	-	327	WP_022923225.1	type II toxin-antitoxin system PemK/MazF family toxin	-
FU792_RS01305	263202..263408	-	207	WP_022926329.1	DUF3018 family protein	-
FU792_RS01310	263796..264473	+	678	WP_149814480.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	247924..271713	23789

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-106)

Antitoxin

• Antitoxin MazE-like (5-66)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11713.53 Da
• Isoelectric Point: 7.0141

>T139233 WP_022923225.1 NZ_CP043808:c259545-259219 [Serinicoccus marinus DSM 15273]
VRRGELWTVAGGVYASKPRPALIVQDDLFEGTASVTVAPLTTTLVDAPLLRVRVAADDFTGLQHDSQVMIDKVTTVRREN
VGTRIGRLAPESLVEVERALMAFLGLAR

Nucleotide

• Length: 327 bp

>T139233 NZ_CP058574:4214397-4214606 [Escherichia coli]
ATGCTGAACACATGTAGAGTGCCTCTTACTGACCGTAAGGTCAAGGAGAAGAGAGCAATGAAGCAGCATAAGGCGATGAT
TGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAAAGACCTCTGTGAGGTTCACATCC
GAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 8055.96 Da
• Isoelectric Point: 5.1425

>AT139233 WP_066638586.1 NZ_CP043808:c259748-259542 [Serinicoccus marinus DSM 15273]
MTVKHRVSEYRRRMRARGLRPVQIWVPDVRSERFAQEAERQAALVARADEQSDDQEFVEAVTAPWDEE

Nucleotide

• Length: 207 bp

>AT139233 NZ_CP058574:c4214405-4214348 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Toxin

• AlphaFold DB: A0A1B1NCA9