Detailed information of TA system TA139212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 482..1125
• Replicon: plasmid unnamed1
• Accession: NZ_CP043768
• Organism: Enterobacter hormaechei strain EB_P9_L5_03.19

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: F1597_RS25980
• Protein ID: WP_016236302.1
• Coordinates: 709..1125 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: F1597_RS25975
• Protein ID: WP_001261282.1
• Coordinates: 482..712 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1597_RS25970	55..525	-	471	Protein_1	hypothetical protein	-
F1597_RS25975	482..712	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
F1597_RS25980	709..1125	+	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
F1597_RS25985	1199..1435	+	237	WP_001348075.1	AAA family ATPase	-
F1597_RS25990	1482..2186	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
F1597_RS25995	2240..2557	+	318	Protein_6	IS110 family transposase	-
F1597_RS26000	2931..3197	+	267	WP_001567383.1	DUF1471 domain-containing protein	-
F1597_RS26005	3294..3851	+	558	WP_001567382.1	TetR/AcrR family transcriptional regulator	-
F1597_RS26010	4064..4324	+	261	WP_001567381.1	DUF1471 domain-containing protein	-
F1597_RS26015	4355..4861	+	507	WP_001567380.1	DUF417 family protein	-
F1597_RS26865	4935..5072	+	138	WP_021243014.1	hypothetical protein	-
F1597_RS26020	5096..5557	+	462	WP_001567378.1	peptide-methionine (R)-S-oxide reductase MsrB	-
F1597_RS26025	5547..6041	+	495	WP_001567377.1	peptide-methionine (S)-S-oxide reductase MsrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..113004	113004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T139212 WP_016236302.1 NZ_CP043768:709-1125 [Enterobacter hormaechei]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T139212 NZ_CP058571:c4094479-4094081 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGAGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT139212 WP_001261282.1 NZ_CP043768:482-712 [Enterobacter hormaechei]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT139212 NZ_CP058571:c4094775-4094482 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3