Detailed information of TA system TA139201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3748655..3749452
• Replicon: chromosome
• Accession: NZ_CP043766
• Organism: Enterobacter hormaechei strain EB_P9_L5_03.19

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: F1597_RS18015
• Protein ID: WP_033487261.1
• Coordinates: 3748931..3749452 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A331QJY7
• Locus tag: F1597_RS18010
• Protein ID: WP_015570876.1
• Coordinates: 3748655..3748924 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1597_RS17975	3744257..3744559	+	303	WP_015570879.1	helix-turn-helix transcriptional regulator	-
F1597_RS17980	3744569..3744733	-	165	WP_072015914.1	helix-turn-helix transcriptional regulator	-
F1597_RS17985	3744888..3746129	+	1242	WP_033487264.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
F1597_RS17990	3746162..3746389	-	228	WP_032670920.1	YccJ family protein	-
F1597_RS17995	3746408..3747004	-	597	WP_033487263.1	NAD(P)H:quinone oxidoreductase	-
F1597_RS18000	3747395..3747565	+	171	WP_001273664.1	general stress protein	-
F1597_RS18005	3747682..3748581	+	900	WP_033487262.1	DMT family transporter	-
F1597_RS18010	3748655..3748924	+	270	WP_015570876.1	DUF1778 domain-containing protein	Antitoxin
F1597_RS18015	3748931..3749452	+	522	WP_033487261.1	GNAT family N-acetyltransferase	Toxin
F1597_RS18020	3749511..3750833	-	1323	WP_032670921.1	pyrimidine utilization transport protein G	-
F1597_RS18025	3750855..3751346	-	492	WP_015570873.1	pyrimidine utilization flavin reductase protein F	-
F1597_RS18030	3751359..3751949	-	591	WP_017693662.1	malonic semialdehyde reductase	-
F1597_RS18035	3751959..3752759	-	801	WP_023296287.1	pyrimidine utilization protein D	-
F1597_RS18040	3752767..3753153	-	387	WP_151574279.1	pyrimidine utilization protein C	-
F1597_RS18045	3753165..3753854	-	690	WP_023296288.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-88)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19453.18 Da
• Isoelectric Point: 7.4318

>T139201 WP_033487261.1 NZ_CP043766:3748931-3749452 [Enterobacter hormaechei]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQS

Nucleotide

• Length: 522 bp

>T139201 NZ_CP058571:1053786-1054121 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10247.75 Da
• Isoelectric Point: 5.8836

>AT139201 WP_015570876.1 NZ_CP043766:3748655-3748924 [Enterobacter hormaechei]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT139201 NZ_CP058571:1053538-1053786 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A331QJY7