Detailed information of TA system TA139197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2604619..2605195
• Replicon: chromosome
• Accession: NZ_CP043766
• Organism: Enterobacter hormaechei strain EB_P9_L5_03.19

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A800YKM1
• Locus tag: F1597_RS12445
• Protein ID: WP_015572580.1
• Coordinates: 2604619..2604906 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: F1597_RS12450
• Protein ID: WP_017694570.1
• Coordinates: 2604893..2605195 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1597_RS12420	2599710..2600429	+	720	WP_050515874.1	winged helix-turn-helix domain-containing protein	-
F1597_RS12425	2600579..2601049	+	471	WP_033486706.1	MarR family transcriptional regulator	-
F1597_RS12430	2601046..2602113	+	1068	WP_026080654.1	HlyD family secretion protein	-
F1597_RS12435	2602103..2603179	+	1077	WP_015572578.1	DUF2955 domain-containing protein	-
F1597_RS12440	2603176..2604447	-	1272	WP_033486707.1	DUF445 domain-containing protein	-
F1597_RS12445	2604619..2604906	+	288	WP_015572580.1	BrnT family toxin	Toxin
F1597_RS12450	2604893..2605195	+	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
F1597_RS12455	2605224..2605862	-	639	WP_033486708.1	LysE family translocator	-
F1597_RS12460	2605901..2606653	-	753	WP_033486709.1	AraC family transcriptional regulator	-
F1597_RS12465	2606807..2608177	+	1371	WP_033486710.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
F1597_RS12470	2608353..2608898	-	546	WP_151574159.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11296.81 Da
• Isoelectric Point: 7.3587

>T139197 WP_015572580.1 NZ_CP043766:2604619-2604906 [Enterobacter hormaechei]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

>T139197 NZ_CP058571:c593916-593620 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT139197 WP_017694570.1 NZ_CP043766:2604893-2605195 [Enterobacter hormaechei]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT139197 NZ_CP043766:2604893-2605195 [Enterobacter hormaechei]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGCGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA