Detailed information of TA system TA139187

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3432689..3433307
• Replicon: chromosome
• Accession: NZ_CP043727
• Organism: Yersinia canariae strain NCTC 14382

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: Q66DR5
• Locus tag: F0T03_RS15905
• Protein ID: WP_002208622.1
• Coordinates: 3433104..3433307 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A857F2E4
• Locus tag: F0T03_RS15900
• Protein ID: WP_004392643.1
• Coordinates: 3432689..3433057 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0T03_RS15870	3428370..3428630	+	261	WP_145555763.1	type B 50S ribosomal protein L31	-
F0T03_RS15875	3428646..3428789	+	144	WP_159679428.1	type B 50S ribosomal protein L36	-
F0T03_RS15880	3428905..3429786	-	882	WP_145555761.1	metal ABC transporter substrate-binding protein	-
F0T03_RS15885	3429815..3430672	-	858	WP_159679429.1	metal ABC transporter permease	-
F0T03_RS15890	3430669..3431406	-	738	WP_159679430.1	ABC transporter ATP-binding protein	-
F0T03_RS15895	3432177..3432530	+	354	WP_159679431.1	hypothetical protein	-
F0T03_RS15900	3432689..3433057	+	369	WP_004392643.1	Hha toxicity modulator TomB	Antitoxin
F0T03_RS15905	3433104..3433307	+	204	WP_002208622.1	expression modulating protein YmoA	Toxin
F0T03_RS15915	3434279..3434584	+	306	WP_208787128.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
F0T03_RS15920	3434635..3435165	-	531	WP_145555756.1	YbaY family lipoprotein	-
F0T03_RS15925	3435423..3436283	+	861	WP_145555755.1	acyl-CoA thioesterase II	-
F0T03_RS15930	3436380..3437669	-	1290	WP_162527046.1	ammonium transporter AmtB	-
F0T03_RS15935	3437709..3438047	-	339	WP_002208627.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8063.31 Da
• Isoelectric Point: 6.4573

>T139187 WP_002208622.1 NZ_CP043727:3433104-3433307 [Yersinia canariae]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPPTVWQHVK

Nucleotide

• Length: 204 bp

>T139187 NZ_CP058549:c64926-64444 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCGGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14176.95 Da
• Isoelectric Point: 4.5140

>AT139187 WP_004392643.1 NZ_CP043727:3432689-3433057 [Yersinia canariae]
MDEYSPKRHDIAQLKFLCESLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYPDGGDLSELVEEYL
DDTYTLFSSYGINDPELRRWQKTKERLFRLFSGEYICTLMKT

Nucleotide

• Length: 369 bp

>AT139187 NZ_CP058549:c65180-64914 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAAATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• PDB: 2K5S

Antitoxin

• AlphaFold DB: A0A857F2E4