Detailed information of TA system TA139168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 45986..46527
• Replicon: plasmid pMaqPC3
• Accession: NZ_CP043630
• Organism: Methylobacterium aquaticum strain BG2

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: F1D61_RS33445
• Protein ID: WP_203159539.1
• Coordinates: 46234..46527 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: F1D61_RS33440
• Protein ID: WP_203159538.1
• Coordinates: 45986..46237 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1D61_RS33415	41412..42692	-	1281	WP_203159534.1	IS66 family transposase	-
F1D61_RS33420	43212..43478	+	267	WP_048450833.1	hypothetical protein	-
F1D61_RS33425	43953..44387	-	435	WP_203159535.1	hypothetical protein	-
F1D61_RS33430	44726..45097	-	372	WP_203159536.1	stability/partitioning determinant	-
F1D61_RS33435	45097..45783	-	687	WP_203159537.1	ParA family protein	-
F1D61_RS33440	45986..46237	+	252	WP_203159538.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
F1D61_RS33445	46234..46527	+	294	WP_203159539.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F1D61_RS33450	46665..46886	+	222	WP_203159540.1	hypothetical protein	-
F1D61_RS33455	48253..51015	-	2763	WP_203159541.1	relaxase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..66072	66072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Antitoxin ParD (4-72)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11137.75 Da
• Isoelectric Point: 8.0503

>T139168 WP_203159539.1 NZ_CP043630:46234-46527 [Methylobacterium aquaticum]
MSGYVLTPAAQADVAEIWDYTAERWSREQATAYIRAIQEACEDLASGMRISRPVDVKPGYLKTNVKSHFVIFRRDDAGLV
TMVRILHSRMDVERHLR

Nucleotide

• Length: 294 bp

>T139168 NZ_CP043630:46234-46527 [Methylobacterium aquaticum]
ATGAGCGGCTACGTCCTTACGCCGGCAGCCCAGGCAGATGTTGCCGAGATCTGGGACTACACAGCAGAGCGGTGGAGCCG
GGAGCAGGCAACAGCCTACATCCGTGCCATTCAGGAGGCCTGCGAGGATTTGGCATCTGGCATGCGGATCAGCCGGCCGG
TCGATGTGAAACCTGGATATCTCAAAACCAACGTCAAATCGCACTTCGTCATATTCAGGCGGGACGACGCTGGGCTGGTT
ACCATGGTCCGTATTCTGCACAGCCGCATGGATGTGGAGCGACATCTCCGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8820.77 Da
• Isoelectric Point: 4.7469

>AT139168 WP_203159538.1 NZ_CP043630:45986-46237 [Methylobacterium aquaticum]
MAKNTSVTLGDHFTGFIDRQVEAGRYGSASEVVRAGLRLLEEHEAKVQALQAAIQAGEESGPATAFDFDAFIASKRAPAS
EPQ

Nucleotide

• Length: 252 bp

>AT139168 NZ_CP058544:4659251-4659625 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG