Detailed information of TA system TA139165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 4407176..4407761
• Replicon: chromosome
• Accession: NZ_CP043627
• Organism: Methylobacterium aquaticum strain BG2

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: F1D61_RS20115
• Protein ID: WP_203159169.1
• Coordinates: 4407477..4407761 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: F1D61_RS20110
• Protein ID: WP_203153532.1
• Coordinates: 4407176..4407496 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F1D61_RS20085	4402184..4402699	+	516	WP_203153523.1	CreA family protein	-
F1D61_RS20090	4402722..4403090	-	369	WP_203153525.1	hypothetical protein	-
F1D61_RS20095	4403159..4405102	-	1944	WP_203153527.1	hypothetical protein	-
F1D61_RS20100	4405209..4405916	-	708	WP_203153529.1	NUDIX hydrolase	-
F1D61_RS20105	4406113..4407030	-	918	WP_203153531.1	PhzF family phenazine biosynthesis protein	-
F1D61_RS20110	4407176..4407496	-	321	WP_203153532.1	BrnA antitoxin family protein	Antitoxin
F1D61_RS20115	4407477..4407761	-	285	WP_203159169.1	BrnT family toxin	Toxin
F1D61_RS20120	4407864..4408868	+	1005	WP_203153533.1	DUF2891 domain-containing protein	-
F1D61_RS20125	4409019..4409867	-	849	WP_203153534.1	pyridoxal kinase PdxY	-
F1D61_RS20130	4410107..4410631	+	525	WP_203153535.1	DedA family protein	-
F1D61_RS20135	4410795..4411952	+	1158	WP_203153536.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (3-85)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (30-102)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10411.62 Da
• Isoelectric Point: 9.8618

>T139165 WP_203159169.1 NZ_CP043627:c4407761-4407477 [Methylobacterium aquaticum]
MVWDEPKRKKNRQPPPAGHGLDFADARDRFEWETAAISESYSGKSGGARYIATGYLDGKLVSLTFSLLGTEAVSAISLRT
ASNRERRAYAGRFE

Nucleotide

• Length: 285 bp

>T139165 NZ_CP058544:2489286-2489388 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11612.30 Da
• Isoelectric Point: 8.7441

>AT139165 WP_203153532.1 NZ_CP043627:c4407496-4407176 [Methylobacterium aquaticum]
MPDDSSKRAVPMLSDAEEAELQARMAADPDAREMTDEELGRMRPAREVLPPAVFAALTKRGRPKSPSPLVQVTLRLDSEA
LAAFKATGPGWQTRINETVKRAAKQL

Nucleotide

• Length: 321 bp

>AT139165 NZ_CP058544:c2489395-2489250 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT