Detailed information of TA system TA139151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 103818..104554
• Replicon: plasmid p1_085031
• Accession: NZ_CP043599
• Organism: Klebsiella pneumoniae strain WCHKP085031

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: B7D33_RS28150
• Protein ID: WP_004187044.1
• Coordinates: 104072..104554 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: B7D33_RS28145
• Protein ID: WP_003026799.1
• Coordinates: 103818..104084 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D33_RS28125 (B7D33_28445)	98967..99947	+	981	WP_023307502.1	IS5-like element ISKpn26 family transposase	-
B7D33_RS28130 (B7D33_28450)	100005..100250	+	246	WP_032238678.1	hypothetical protein	-
B7D33_RS28135 (B7D33_28455)	100219..102804	+	2586	WP_004187040.1	EAL domain-containing protein	-
B7D33_RS28140 (B7D33_28460)	102963..103667	-	705	WP_060579418.1	IS6-like element IS26 family transposase	-
B7D33_RS28145 (B7D33_28470)	103818..104084	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
B7D33_RS28150 (B7D33_28475)	104072..104554	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
B7D33_RS28155 (B7D33_28480)	104754..106157	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
B7D33_RS28160 (B7D33_28485)	106186..106818	-	633	WP_001567369.1	hypothetical protein	-
B7D33_RS28165 (B7D33_28495)	107220..108107	+	888	WP_227521777.1	IS110 family transposase	-
B7D33_RS28170 (B7D33_28500)	108230..108670	-	441	WP_142255031.1	NirD/YgiW/YdeI family stress tolerance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..142761	142761
	-	inside	IScluster/Tn	-	-	98967..106157	7190

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T139151 WP_004187044.1 NZ_CP043599:104072-104554 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T139151 NZ_CP058410:c235187-234936 [Trichlorobacter lovleyi]
ATGAATGCCCTGACCTATACCTATACGCGCCAGCATTTTGCAGAGGTCATGAAATCAGTAAATGAAGACCACGTTCCCGT
GGTGGTGACCAGCCAGCGCGGCAAACCGGTTGTCATCCTTTCCCTCGACGATTTCCACGCTTATGAAGAAACCGCCTATC
TGCTCCGTAATCCCCAAGGGGCCAAACGGTTGCTTGAATCTGTGGAGGAACTGCGTGCAGGCGGAGGGCAGACCAGAGAC
CTGACGGAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT139151 WP_003026799.1 NZ_CP043599:103818-104084 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT139151 NZ_CP058410:c234939-234685 [Trichlorobacter lovleyi]
ATGATGATAAAATTCTCAACCGCAGCATGGGAGGACTATCTCTACTGGCAGACTACCGACAAGGCGATGCTGAAACGGAT
CAATCAACTTATCCGGGATATCCAGCGAGAACCGTTTGCCGGTATCGGCAAGCCGGAACCGCTCAAACATCAATTGGCCG
GTTTCTGGTCACGCAGAATAGACAGCTGCCACCGCTTGGTCTATGCGGTTGAAGGTGATACGCTCCTGATTGCACAGTGT
AGAGATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66