Detailed information of TA system TA139150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 90401..90923
• Replicon: plasmid p1_085031
• Accession: NZ_CP043599
• Organism: Klebsiella pneumoniae strain WCHKP085031

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A9J6S4Z8
• Locus tag: B7D33_RS28055
• Protein ID: WP_004187019.1
• Coordinates: 90401..90685 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4ZSR4
• Locus tag: B7D33_RS28060
• Protein ID: WP_004187025.1
• Coordinates: 90675..90923 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D33_RS28035 (B7D33_28350)	85620..86084	-	465	WP_004187010.1	conjugative transfer protein MobI(A/C)	-
B7D33_RS28040 (B7D33_28360)	87627..88040	+	414	WP_227521778.1	hypothetical protein	-
B7D33_RS28045	88697..88948	+	252	Protein_86	conjugal transfer protein TraH	-
B7D33_RS28050 (B7D33_28370)	89030..90385	+	1356	WP_004187017.1	ISNCY family transposase	-
B7D33_RS28055 (B7D33_28375)	90401..90685	-	285	WP_004187019.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B7D33_RS28060 (B7D33_28380)	90675..90923	-	249	WP_004187025.1	plasmid stabilization protein	Antitoxin
B7D33_RS28065 (B7D33_28385)	91213..91637	-	425	Protein_90	IS1 family transposase	-
B7D33_RS28070 (B7D33_28390)	91825..91941	-	117	Protein_91	transposase domain-containing protein	-
B7D33_RS28075 (B7D33_28395)	91886..92149	-	264	WP_004187027.1	transposase	-
B7D33_RS28080 (B7D33_28400)	92164..92427	+	264	WP_004118208.1	hypothetical protein	-
B7D33_RS28085 (B7D33_28405)	92671..92952	+	282	WP_013307885.1	helix-turn-helix domain-containing protein	-
B7D33_RS28090 (B7D33_28410)	92987..93556	+	570	WP_004152117.1	small heat shock protein sHSP20	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..142761	142761
	-	flank	IS/Tn	-	-	91177..91443	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11003.72 Da
• Isoelectric Point: 10.5388

>T139150 WP_004187019.1 NZ_CP043599:c90685-90401 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T139150 NZ_CP058410:c234939-234685 [Trichlorobacter lovleyi]
ATGATGATAAAATTCTCAACCGCAGCATGGGAGGACTATCTCTACTGGCAGACTACCGACAAGGCGATGCTGAAACGGAT
CAATCAACTTATCCGGGATATCCAGCGAGAACCGTTTGCCGGTATCGGCAAGCCGGAACCGCTCAAACATCAATTGGCCG
GTTTCTGGTCACGCAGAATAGACAGCTGCCACCGCTTGGTCTATGCGGTTGAAGGTGATACGCTCCTGATTGCACAGTGT
AGAGATCATTATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9011.24 Da
• Isoelectric Point: 3.9947

>AT139150 WP_004187025.1 NZ_CP043599:c90923-90675 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT139150 NZ_CP058410:c235187-234936 [Trichlorobacter lovleyi]
ATGAATGCCCTGACCTATACCTATACGCGCCAGCATTTTGCAGAGGTCATGAAATCAGTAAATGAAGACCACGTTCCCGT
GGTGGTGACCAGCCAGCGCGGCAAACCGGTTGTCATCCTTTCCCTCGACGATTTCCACGCTTATGAAGAAACCGCCTATC
TGCTCCGTAATCCCCAAGGGGCCAAACGGTTGCTTGAATCTGTGGAGGAACTGCGTGCAGGCGGAGGGCAGACCAGAGAC
CTGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4ZSR4