Detailed information of TA system TA139149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 182300..183057
• Replicon: plasmid pNDM1_085031
• Accession: NZ_CP043598
• Organism: Klebsiella pneumoniae strain WCHKP085031

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J4ZXV7
• Locus tag: B7D33_RS26455
• Protein ID: WP_029497408.1
• Coordinates: 182300..182782 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: B7D33_RS26460
• Protein ID: WP_094645258.1
• Coordinates: 182770..183057 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D33_RS26425 (B7D33_26615)	177331..177546	-	216	WP_009653220.1	hypothetical protein	-
B7D33_RS26430 (B7D33_26620)	179081..179458	+	378	WP_042927811.1	hypothetical protein	-
B7D33_RS26435 (B7D33_26625)	179821..180792	-	972	WP_009653249.1	hypothetical protein	-
B7D33_RS26440 (B7D33_26630)	181332..181592	+	261	WP_009653224.1	hypothetical protein	-
B7D33_RS26445 (B7D33_26635)	181800..181922	-	123	WP_223256846.1	type I toxin-antitoxin system Hok family toxin	-
B7D33_RS26450	181867..182019	-	153	Protein_181	DUF5431 family protein	-
B7D33_RS26455 (B7D33_26640)	182300..182782	-	483	WP_029497408.1	GNAT family N-acetyltransferase	Toxin
B7D33_RS26460 (B7D33_26645)	182770..183057	-	288	WP_094645258.1	DUF1778 domain-containing protein	Antitoxin
B7D33_RS26465 (B7D33_26650)	183294..184217	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
B7D33_RS26470 (B7D33_26655)	184417..184989	-	573	WP_094645259.1	cytochrome b/b6 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / sul1 / blaNDM-1 / qacE / aadA2 / dfrA12 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / catA2 / tet(D) / blaCTX-M-14 / ARR-3 / dfrA27 / aadA16 / mph(A)	katA	1..401561	401561
	-	flank	IS/Tn	-	-	185515..186318	803

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17599.34 Da
• Isoelectric Point: 9.8719

>T139149 WP_029497408.1 NZ_CP043598:c182782-182300 [Klebsiella pneumoniae]
VGRLTAPEPLSAFHQVAEFVSGEAVLDDWLKQKGLKNQALGAARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFHGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLRLP

Nucleotide

• Length: 483 bp

>T139149 NZ_CP058410:c172991-172749 [Trichlorobacter lovleyi]
CTGACCTATACCTATACGCGCCAGCATTTTGCAGAGGTCATGAAATCAGTAAATGAAGACCACGTTCCCGTGGTGGTGAC
CAGCCAGCGCGGCAAACCGGTTGTCATCCTTTCCCTCGACGATTTCCACGCTTATGAAGAAACCGCCTATCTGCTCCGTA
ATCCCCAAGGGGCCAAACGGTTGCTTGAATCTGTGGAGGAACTGCGTGCAGGCGGAGGGCAGACCAGAGACCTGACGGAA
TGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10955.66 Da
• Isoelectric Point: 4.5141

>AT139149 WP_094645258.1 NZ_CP043598:c183057-182770 [Klebsiella pneumoniae]
MLYKGCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVVDD
PVIEKLLARKPQWDV

Nucleotide

• Length: 288 bp

>AT139149 NZ_CP043598:c183057-182770 [Klebsiella pneumoniae]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGCGGAGTTCATCGACATGCTTGATGCACCTGTCGTAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTTTAA

Structures

Toxin

• AlphaFold DB: A0A2J4ZXV7