Detailed information of TA system TA139134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1202285..1202904
• Replicon: chromosome
• Accession: NZ_CP043597
• Organism: Klebsiella pneumoniae strain WCHKP085031

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: B7D33_RS05845
• Protein ID: WP_002892050.1
• Coordinates: 1202285..1202503 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: J2DPF6
• Locus tag: B7D33_RS05850
• Protein ID: WP_002892066.1
• Coordinates: 1202530..1202904 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7D33_RS05810 (1198332)	1198332..1198595	+	264	WP_002892011.1	type B 50S ribosomal protein L31	-
B7D33_RS05815 (1198595)	1198595..1198735	+	141	WP_040245625.1	type B 50S ribosomal protein L36	-
B7D33_RS05820 (1198732)	1198732..1199430	-	699	WP_002892021.1	GNAT family protein	-
B7D33_RS05825 (1199531)	1199531..1200982	+	1452	WP_002892023.1	PLP-dependent aminotransferase family protein	-
B7D33_RS05830 (1200957)	1200957..1201427	-	471	WP_002892026.1	YlaC family protein	-
B7D33_RS05835 (1201448)	1201448..1201588	+	141	WP_004147370.1	hypothetical protein	-
B7D33_RS05840 (1201560)	1201560..1202126	-	567	WP_002892030.1	maltose O-acetyltransferase	-
B7D33_RS05845 (1202285)	1202285..1202503	-	219	WP_002892050.1	HHA domain-containing protein	Toxin
B7D33_RS05850 (1202530)	1202530..1202904	-	375	WP_002892066.1	Hha toxicity modulator TomB	Antitoxin
B7D33_RS05855 (1203390)	1203390..1206536	-	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
B7D33_RS05860 (1206559)	1206559..1207752	-	1194	WP_004177236.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T139134 WP_002892050.1 NZ_CP043597:c1202503-1202285 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T139134 NZ_CP058361:c5286926-5286543 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14368.06 Da
• Isoelectric Point: 4.8989

>AT139134 WP_002892066.1 NZ_CP043597:c1202904-1202530 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT139134 NZ_CP043597:c1202904-1202530 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2

Antitoxin

• AlphaFold DB: A0A0H3GJ93