Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1202285..1202904 Replicon chromosome
Accession NZ_CP043597
Organism Klebsiella pneumoniae strain WCHKP085031

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag B7D33_RS05845 Protein ID WP_002892050.1
Coordinates 1202285..1202503 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag B7D33_RS05850 Protein ID WP_002892066.1
Coordinates 1202530..1202904 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D33_RS05810 (1198332) 1198332..1198595 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
B7D33_RS05815 (1198595) 1198595..1198735 + 141 WP_040245625.1 type B 50S ribosomal protein L36 -
B7D33_RS05820 (1198732) 1198732..1199430 - 699 WP_002892021.1 GNAT family protein -
B7D33_RS05825 (1199531) 1199531..1200982 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
B7D33_RS05830 (1200957) 1200957..1201427 - 471 WP_002892026.1 YlaC family protein -
B7D33_RS05835 (1201448) 1201448..1201588 + 141 WP_004147370.1 hypothetical protein -
B7D33_RS05840 (1201560) 1201560..1202126 - 567 WP_002892030.1 maltose O-acetyltransferase -
B7D33_RS05845 (1202285) 1202285..1202503 - 219 WP_002892050.1 HHA domain-containing protein Toxin
B7D33_RS05850 (1202530) 1202530..1202904 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
B7D33_RS05855 (1203390) 1203390..1206536 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
B7D33_RS05860 (1206559) 1206559..1207752 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T139134 WP_002892050.1 NZ_CP043597:c1202503-1202285 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T139134 NZ_CP058361:c5286926-5286543 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT139134 WP_002892066.1 NZ_CP043597:c1202904-1202530 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT139134 NZ_CP043597:c1202904-1202530 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References