Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 7394..7919 Replicon plasmid pNDM1_015096
Accession NZ_CP043589
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP015096

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag CLR01_RS25395 Protein ID WP_008322213.1
Coordinates 7394..7699 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag CLR01_RS25400 Protein ID WP_032934863.1
Coordinates 7701..7919 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLR01_RS25365 (CLR01_25520) 2531..2647 + 117 Protein_2 IS5 family transposase -
CLR01_RS25370 (CLR01_25530) 2719..3839 + 1121 WP_089046468.1 IS3-like element ISSen4 family transposase -
CLR01_RS25375 (CLR01_25535) 3868..4614 + 747 Protein_4 IS5-like element IS5 family transposase -
CLR01_RS25380 (CLR01_25540) 4776..6098 + 1323 WP_016479957.1 GntP family transporter -
CLR01_RS25385 (CLR01_25545) 6297..7088 - 792 WP_008322208.1 site-specific integrase -
CLR01_RS25390 (CLR01_25550) 7078..7392 - 315 WP_008322211.1 hypothetical protein -
CLR01_RS25395 (CLR01_25555) 7394..7699 - 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
CLR01_RS25400 (CLR01_25560) 7701..7919 - 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
CLR01_RS25405 (CLR01_25565) 8781..9478 - 698 Protein_10 IS1-like element IS1B family transposase -
CLR01_RS25410 (CLR01_25570) 9691..12273 + 2583 WP_016479955.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 htpB 1..99510 99510
- inside IScluster/Tn - - 2531..24519 21988


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T139110 WP_008322213.1 NZ_CP043589:c7699-7394 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T139110 NZ_CP058356:30184-30705 [Escherichia coli]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAACGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCACT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAAGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAACCTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGAATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAGGGGCT
TGGCTCAATGCTTGTAACTCACGCAATGCGTGTTGTGTACAATGCATCTCTTGCGGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTGAACGATAAAGCCAGGGCGTTTTATAAAAGTTTGGGCTTTATCCAGCTGGTTGGTAACAACGATCGTTCTTTG
TTCTATCCAACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT139110 WP_032934863.1 NZ_CP043589:c7919-7701 [Klebsiella pneumoniae subsp. pneumoniae]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT139110 NZ_CP058356:29907-30176 [Escherichia coli]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACCCAGTTCATGGTTAGCAGTGCATCCGAACGTGCCGTGAAGGTGATAGAGCAACACCGTAGAC
TGGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References