Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5119048..5119673 Replicon chromosome
Accession NZ_CP043588
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP015096

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag CLR01_RS24990 Protein ID WP_002882817.1
Coordinates 5119048..5119431 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag CLR01_RS24995 Protein ID WP_004150355.1
Coordinates 5119431..5119673 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLR01_RS24975 (5116414) 5116414..5117316 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
CLR01_RS24980 (5117313) 5117313..5117948 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
CLR01_RS24985 (5117945) 5117945..5118874 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
CLR01_RS24990 (5119048) 5119048..5119431 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
CLR01_RS24995 (5119431) 5119431..5119673 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
CLR01_RS25000 (5119878) 5119878..5120795 + 918 WP_065803683.1 alpha/beta hydrolase -
CLR01_RS25005 (5120809) 5120809..5121750 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
CLR01_RS25010 (5121795) 5121795..5122232 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
CLR01_RS25015 (5122229) 5122229..5123089 - 861 WP_004146232.1 virulence factor BrkB family protein -
CLR01_RS25020 (5123083) 5123083..5123682 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T139109 WP_002882817.1 NZ_CP043588:c5119431-5119048 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T139109 NZ_CP058355:c4152059-4151886 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT139109 WP_004150355.1 NZ_CP043588:c5119673-5119431 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT139109 NZ_CP058355:c4152383-4152087 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References