Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3918436..3919055 Replicon chromosome
Accession NZ_CP043588
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP015096

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CLR01_RS19275 Protein ID WP_002892050.1
Coordinates 3918837..3919055 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CLR01_RS19270 Protein ID WP_002892066.1
Coordinates 3918436..3918810 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLR01_RS19260 (3913588) 3913588..3914781 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CLR01_RS19265 (3914804) 3914804..3917950 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CLR01_RS19270 (3918436) 3918436..3918810 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CLR01_RS19275 (3918837) 3918837..3919055 + 219 WP_002892050.1 HHA domain-containing protein Toxin
CLR01_RS19280 (3919214) 3919214..3919780 + 567 WP_002892030.1 maltose O-acetyltransferase -
CLR01_RS19285 (3919752) 3919752..3919892 - 141 WP_004147370.1 hypothetical protein -
CLR01_RS19290 (3919913) 3919913..3920383 + 471 WP_002892026.1 YlaC family protein -
CLR01_RS19295 (3920358) 3920358..3921809 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
CLR01_RS19300 (3921910) 3921910..3922608 + 699 WP_004177238.1 GNAT family protein -
CLR01_RS19305 (3922605) 3922605..3922745 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CLR01_RS19310 (3922745) 3922745..3923008 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T139105 WP_002892050.1 NZ_CP043588:3918837-3919055 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T139105 NZ_CP058355:2633732-2633839 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT139105 WP_002892066.1 NZ_CP043588:3918436-3918810 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT139105 NZ_CP043588:3918436-3918810 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References