Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2564075..2565663 Replicon chromosome
Accession NZ_CP043588
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP015096

Toxin (Protein)


Gene name hipA Uniprot ID A0A486RAG1
Locus tag CLR01_RS12630 Protein ID WP_016946382.1
Coordinates 2564075..2565397 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag CLR01_RS12635 Protein ID WP_002904790.1
Coordinates 2565397..2565663 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLR01_RS12615 (2559131) 2559131..2561632 + 2502 WP_023282574.1 fimbria/pilus outer membrane usher protein -
CLR01_RS12620 (2561620) 2561620..2562624 + 1005 WP_016946380.1 fimbrial protein -
CLR01_RS12625 (2562766) 2562766..2564058 + 1293 WP_016946381.1 glycoside hydrolase family 10 protein -
CLR01_RS12630 (2564075) 2564075..2565397 - 1323 WP_016946382.1 type II toxin-antitoxin system HipA family toxin Toxin
CLR01_RS12635 (2565397) 2565397..2565663 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
CLR01_RS12640 (2566050) 2566050..2566376 - 327 WP_004215084.1 hydroxyisourate hydrolase -
CLR01_RS12645 (2566373) 2566373..2566873 - 501 WP_023282573.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
CLR01_RS12650 (2566851) 2566851..2568230 - 1380 WP_004184021.1 nucleobase:cation symporter-2 family protein -
CLR01_RS12655 (2568576) 2568576..2569730 + 1155 WP_023282572.1 FAD-dependent urate hydroxylase HpxO -
CLR01_RS12660 (2569711) 2569711..2570634 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49125.44 Da        Isoelectric Point: 7.5358

>T139104 WP_016946382.1 NZ_CP043588:c2565397-2564075 [Klebsiella pneumoniae subsp. pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T139104 NZ_CP058355:2633197-2633304 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT139104 WP_002904790.1 NZ_CP043588:c2565663-2565397 [Klebsiella pneumoniae subsp. pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT139104 NZ_CP058355:c2633150-2633084 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486RAG1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References