Detailed information of TA system TA139092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 16101..16744
• Replicon: plasmid p1_020151
• Accession: NZ_CP043587
• Organism: Klebsiella pneumoniae subsp. ozaenae strain SCKP020151

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CLQ49_RS25965
• Protein ID: WP_023345587.1
• Coordinates: 16328..16744 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: CLQ49_RS25960
• Protein ID: WP_023345588.1
• Coordinates: 16101..16331 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ49_RS25935 (CLQ49_26095)	12193..12621	-	429	WP_023345594.1	DUF4440 domain-containing protein	-
CLQ49_RS25940 (CLQ49_26100)	12734..13579	-	846	WP_048265004.1	SDR family oxidoreductase	-
CLQ49_RS25945 (CLQ49_26105)	13730..14077	+	348	WP_048265031.1	helix-turn-helix domain-containing protein	-
CLQ49_RS25950 (CLQ49_26110)	14189..14773	+	585	WP_023345591.1	TetR/AcrR family transcriptional regulator	-
CLQ49_RS25955 (CLQ49_26115)	14841..15683	+	843	WP_048265003.1	nitroreductase family protein	-
CLQ49_RS25960 (CLQ49_26120)	16101..16331	+	231	WP_023345588.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CLQ49_RS25965 (CLQ49_26125)	16328..16744	+	417	WP_023345587.1	type II toxin-antitoxin system VapC family toxin	Toxin
CLQ49_RS25970 (CLQ49_26130)	16836..18239	-	1404	WP_023345586.1	glycoside hydrolase family 1 protein	-
CLQ49_RS25975 (CLQ49_26135)	18249..20123	-	1875	WP_048265001.1	beta-glucoside-specific PTS transporter subunit IIABC	-
CLQ49_RS25980 (CLQ49_26140)	20513..21148	-	636	WP_023345584.1	DUF1349 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iroN / ybtS / ybtX / ybtQ / ybtP / ybtA / irp2 / irp1 / ybtU / ybtT / ybtE / fyuA	1..163125	163125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-48)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15082.55 Da
• Isoelectric Point: 7.8644

>T139092 WP_023345587.1 NZ_CP043587:16328-16744 [Klebsiella pneumoniae subsp. ozaenae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLMLEDWVN

Nucleotide

• Length: 417 bp

>T139092 NZ_CP058355:c49492-49115 [Escherichia coli]
ATGAACACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT139092 WP_023345588.1 NZ_CP043587:16101-16331 [Klebsiella pneumoniae subsp. ozaenae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFAP

Nucleotide

• Length: 231 bp

>AT139092 NZ_CP058355:c49950-49582 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA