Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5197739..5198364 Replicon chromosome
Accession NZ_CP043585
Organism Klebsiella pneumoniae subsp. ozaenae strain SCKP020151

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag CLQ49_RS25050 Protein ID WP_002882817.1
Coordinates 5197739..5198122 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag CLQ49_RS25055 Protein ID WP_004150355.1
Coordinates 5198122..5198364 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ49_RS25035 (CLQ49_25170) 5195105..5196007 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
CLQ49_RS25040 (CLQ49_25175) 5196004..5196639 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
CLQ49_RS25045 (CLQ49_25180) 5196636..5197565 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
CLQ49_RS25050 (CLQ49_25185) 5197739..5198122 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
CLQ49_RS25055 (CLQ49_25190) 5198122..5198364 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
CLQ49_RS25060 (CLQ49_25195) 5198569..5199486 + 918 WP_004895173.1 alpha/beta hydrolase -
CLQ49_RS25065 (CLQ49_25200) 5199500..5200441 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
CLQ49_RS25070 (CLQ49_25205) 5200486..5200923 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
CLQ49_RS25075 (CLQ49_25210) 5200920..5201780 - 861 WP_004146232.1 virulence factor BrkB family protein -
CLQ49_RS25080 (CLQ49_25215) 5201774..5202373 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T139091 WP_002882817.1 NZ_CP043585:c5198122-5197739 [Klebsiella pneumoniae subsp. ozaenae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T139091 NZ_CP058354:c2444697-2444272 [Bradyrhizobium japonicum]
GTGAACCTGCTCCTCGACACCAACGTGCTGTCGGAGGTTCAGCGGCCGGCGCCTTCCCCGAAAGTCCTGGCATGGCTCGA
TACGGTCGATGAGGATCGCGCGTTCATCAGCGTTGCCTCGATCGCCGAGCTTCGCCGCGGCATCGCCCTGCTGGATGATG
GCCGTCGCCGCGCCGCACTGGCCGCTTGGCTCGCGCATGACCTGCCGGCTCGGTTCGCGCAGCGCACCCTGCCGATCGAC
CACGCGGTCGCCGAGCGCTGGGGCGACCTGATGGCGCAGAGCCGTCGCACTGGCGTTGCGTTGTCCGTGATGGACGGCTT
CCTTGCGGCCACCGCGTTCGCCAACAATCTCACGCTCGTCACACGCAATGTGACGGATTTCGCGGCCTTCGGCGTTCCGC
TGCTCAACCCGTGGAGCGGCGCCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT139091 WP_004150355.1 NZ_CP043585:c5198364-5198122 [Klebsiella pneumoniae subsp. ozaenae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT139091 NZ_CP058354:c2444975-2444694 [Bradyrhizobium japonicum]
ATGACAGACCGCAGCTCCGCTCCCGATTCGCCGCCGACTGACACGTGGACGCTCGCCAATGCCAAGGCACGCCTGTCCGA
GGTGGTCGATCGCGCCCAAACTGGTCCCCAGATCATCACCCGACACGGCAGGCCCAATGCCGTGGTCGTTTCCGCGGATG
AATGGGCGCGAAAGACGGCGCGCAAAGGCACGCTGGCCGAATTCCTGCTGGCTTCGCCGCTGCGCGGTGCCGACCTCGAA
CTTGAACGGGTGCAGGATGAACCGCGCGACGAGATGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References