Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4020871..4021490 Replicon chromosome
Accession NZ_CP043585
Organism Klebsiella pneumoniae subsp. ozaenae strain SCKP020151

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag CLQ49_RS19465 Protein ID WP_002892050.1
Coordinates 4021272..4021490 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag CLQ49_RS19460 Protein ID WP_002892066.1
Coordinates 4020871..4021245 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ49_RS19450 (CLQ49_19560) 4016023..4017216 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
CLQ49_RS19455 (CLQ49_19565) 4017239..4020385 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
CLQ49_RS19460 (CLQ49_19570) 4020871..4021245 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
CLQ49_RS19465 (CLQ49_19575) 4021272..4021490 + 219 WP_002892050.1 HHA domain-containing protein Toxin
CLQ49_RS19470 (CLQ49_19580) 4021649..4022215 + 567 WP_002892030.1 maltose O-acetyltransferase -
CLQ49_RS19475 4022187..4022327 - 141 WP_032409038.1 hypothetical protein -
CLQ49_RS19480 (CLQ49_19585) 4022348..4022818 + 471 WP_002892026.1 YlaC family protein -
CLQ49_RS19485 (CLQ49_19590) 4022793..4024244 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
CLQ49_RS19490 (CLQ49_19595) 4024345..4025043 + 699 WP_004893822.1 GNAT family protein -
CLQ49_RS19495 (CLQ49_19600) 4025040..4025180 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
CLQ49_RS19500 (CLQ49_19605) 4025180..4025443 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T139088 WP_002892050.1 NZ_CP043585:4021272-4021490 [Klebsiella pneumoniae subsp. ozaenae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T139088 NZ_CP043585:4021272-4021490 [Klebsiella pneumoniae subsp. ozaenae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT139088 WP_002892066.1 NZ_CP043585:4020871-4021245 [Klebsiella pneumoniae subsp. ozaenae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT139088 NZ_CP058353:c4145315-4145049 [Serratia marcescens]
ATGGATATTAACAACAAAGCACGTATTCACTGGGCTTGCCGTCGTGGCATGCGCGAGCTGGACATCTCCATCATGCCGTT
CTTCGAATACGAATACGACAGCCTGAATGACGCGGACAAGGCGCTGTTTATCCGCCTGCTGGAGTGTGACGATCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCGCCGCAGGACGGCGAGCTGCAGAGAATGGTGACCCTGATCCAAACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References