Detailed information of TA system TA139073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 1670..2250
• Replicon: plasmid p1_020018
• Accession: NZ_CP043584
• Organism: Klebsiella pneumoniae strain SCKP020018

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: C6M74_RS28535
• Protein ID: WP_071177730.1
• Coordinates: 1936..2250 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: C6M74_RS28530
• Protein ID: WP_000093040.1
• Coordinates: 1670..1948 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6M74_RS28515 (C6M74_28950)	243..488	+	246	WP_032440458.1	hypothetical protein	-
C6M74_RS28520 (C6M74_28955)	762..1133	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
C6M74_RS28525	1130..1495	+	366	WP_072354022.1	TonB family protein	-
C6M74_RS28530 (C6M74_28965)	1670..1948	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
C6M74_RS28535 (C6M74_28970)	1936..2250	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C6M74_RS28540 (C6M74_28975)	2414..2842	+	429	WP_001140599.1	hypothetical protein	-
C6M74_RS28545 (C6M74_28980)	2868..3047	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
C6M74_RS28550 (C6M74_28985)	3074..3604	-	531	WP_071177729.1	hypothetical protein	-
C6M74_RS28555 (C6M74_28990)	3611..4342	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
C6M74_RS28560 (C6M74_28995)	4342..6306	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T139073 WP_071177730.1 NZ_CP043584:1936-2250 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T139073 NZ_CP043584:1936-2250 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT139073 WP_000093040.1 NZ_CP043584:1670-1948 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT139073 NZ_CP058350:c1672196-1671972 [Rhizobium sp. ADMK78]
ATGTGGACGATCTCAGACAGCGTAGCACTTGGCCAGCTGATCCATGCTGAGCGCAAGCGTCAGCAACTTACCCAGGAGCA
ACTTGCCGGCGTCGCGGGCGTGGGCGTTCGGTTCGTGCGCGAGCTCGAAAGCGGCAAGGAAAGCTGTCGTGTCGGACTTG
CTTTGACCGTCCTTCAGACGCTCGGCTTGACCGTGAACATCACCAACCGCGGAGCTTCCTCATGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1