Detailed information of TA system TA139071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 154993..155663
• Replicon: plasmid pVir_020018
• Accession: NZ_CP043581
• Organism: Klebsiella pneumoniae strain SCKP020018

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: C6M74_RS27110
• Protein ID: WP_004213072.1
• Coordinates: 154993..155436 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: C6M74_RS27115
• Protein ID: WP_004213073.1
• Coordinates: 155433..155663 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6M74_RS27075 (C6M74_27365)	150405..150680	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
C6M74_RS27080 (C6M74_27370)	150743..151234	+	492	WP_011251327.1	DM13 domain-containing protein	-
C6M74_RS27085 (C6M74_27375)	151283..152203	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
C6M74_RS27090	152294..152697	+	404	Protein_166	GAF domain-containing protein	-
C6M74_RS27095	153215..153849	-	635	Protein_167	mucoid phenotype regulator RmpA2	-
C6M74_RS27100 (C6M74_27405)	154266..154570	+	305	Protein_168	transposase	-
C6M74_RS27105	154593..154844	-	252	WP_186987481.1	hypothetical protein	-
C6M74_RS27110 (C6M74_27415)	154993..155436	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
C6M74_RS27115 (C6M74_27420)	155433..155663	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
C6M74_RS27120 (C6M74_27425)	156271..157404	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
C6M74_RS27125 (C6M74_27430)	157420..157713	+	294	WP_004213076.1	hypothetical protein	-
C6M74_RS27130 (C6M74_27435)	157703..157909	-	207	WP_004213077.1	hypothetical protein	-
C6M74_RS27135 (C6M74_27440)	158261..158551	+	291	WP_004213078.1	hypothetical protein	-
C6M74_RS27140 (C6M74_27445)	158541..159440	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA	1..229186	229186

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T139071 WP_004213072.1 NZ_CP043581:c155436-154993 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T139071 NZ_CP058350:c1423026-1422622 [Rhizobium sp. ADMK78]
ATGGGCGGTAGGTTCGTCGATACGAACGTACTGCTTAACCTCGCTTCTGGCGATGCACAGAGAGCTGATCGTGCCGAGGC
CGTGATTGCTGAAGGCGGCATCATCAGCGTTCAGGTCCTCAATGAGATCGCCAATGTGCTACGCCGCAAGATGCGCATGC
CCTGGCCTGACACGCATCTGTTTCTGGATATGATCCGTGCACTGCTGACCGTAGAGCCGGTCACTTGGGAGACACACGAA
ACTGGATTGCGGATCGCCGAGCGCTACGGGCTTTCCATCTACGACGCAATGATCGTTTCAGCAGCATTGATGGCTGGGTG
CGACACCCTGATGTCCGAAGATATGCAAGACGGTCTTGTCATCGATGACCGCCTGCGCATCGTCAATCCCTTCGGCCAAG
CATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT139071 WP_004213073.1 NZ_CP043581:c155663-155433 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT139071 NZ_CP058350:c1423246-1423016 [Rhizobium sp. ADMK78]
ATGCAGATCGCCAAATGGGGCAACAGCCTTGCGGTGCGTCTTCCCGCCAGCGTGGTCGAAGCGCTGAAGCTGAAGGAAGG
CGATGATATCGAAATTCGCGCCGCAGACTGGCGCGAACTTGATGTGGCGCGAAAGCCTACGCCTGAAGATCTGTTAGAGC
GCCTTCGAGCCTTCCGGGGACGGTTACCGCCCGACTTCAAATTCGACCGGTATGAGGCGAATGGGCGGTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620