Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5384890..5385515 Replicon chromosome
Accession NZ_CP043580
Organism Klebsiella pneumoniae strain SCKP020018

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag C6M74_RS25905 Protein ID WP_012737091.1
Coordinates 5384890..5385273 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag C6M74_RS25910 Protein ID WP_004150355.1
Coordinates 5385273..5385515 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M74_RS25890 (5382256) 5382256..5383158 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
C6M74_RS25895 (5383155) 5383155..5383790 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
C6M74_RS25900 (5383787) 5383787..5384716 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
C6M74_RS25905 (5384890) 5384890..5385273 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
C6M74_RS25910 (5385273) 5385273..5385515 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
C6M74_RS25915 (5385720) 5385720..5386637 + 918 WP_012737090.1 alpha/beta hydrolase -
C6M74_RS25920 (5386651) 5386651..5387592 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
C6M74_RS25925 (5387637) 5387637..5388074 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
C6M74_RS25930 (5388071) 5388071..5388931 - 861 WP_004146232.1 virulence factor BrkB family protein -
C6M74_RS25935 (5388925) 5388925..5389524 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T139068 WP_012737091.1 NZ_CP043580:c5385273-5384890 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T139068 NZ_CP043580:c5385273-5384890 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT139068 WP_004150355.1 NZ_CP043580:c5385515-5385273 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT139068 NZ_CP058350:32425-32823 [Rhizobium sp. ADMK78]
ATGCGATATTACGTGGCTTTGGTCTATAAGGACCCGGAGAGTGCCTACGGCATCAGCTTCCCTGATTTGCCATCCGTATT
CTCGGCAGCGGACCGTGACGAAGACCTTGTCATCAAAGCAATCGAGGCCTTGAGGCTTTGGGCCGAGGATGAAGCCCTGC
CATTACCATCATCGCATCAAGCAATTCTGGCCCGTCACGACATCCAAGCAGAGTTGGCTTCGGGTGCATTCCTGATCCGC
GTGCCTTTGATCGAAGATGACACCCGCACCGTGAGAGCCAATATCACGATGGACGCCGGCACACTGGCTGCCATTGATCA
AGCGGCGAAACAGCGAGGTCTGACGCGCTCTGCCTTTCTCGCGAGCTGCGCACGCAAAGAGATCGAGCGTTCCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References