Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4747348..4748158 Replicon chromosome
Accession NZ_CP043580
Organism Klebsiella pneumoniae strain SCKP020018

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag C6M74_RS22930 Protein ID WP_014908042.1
Coordinates 4747348..4747881 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag C6M74_RS22935 Protein ID WP_002887278.1
Coordinates 4747892..4748158 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M74_RS22925 (4746179) 4746179..4747300 + 1122 WP_012737335.1 cupin domain-containing protein -
C6M74_RS22930 (4747348) 4747348..4747881 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
C6M74_RS22935 (4747892) 4747892..4748158 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
C6M74_RS22940 (4748261) 4748261..4749694 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
C6M74_RS22945 (4749684) 4749684..4750367 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
C6M74_RS22950 (4750539) 4750539..4751924 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
C6M74_RS22955 (4751942) 4751942..4752286 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T139066 WP_014908042.1 NZ_CP043580:c4747881-4747348 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T139066 NZ_CP058349:1300588-1300941 [Pseudomonas sp. ABC1]
ATGATGAAAACGCTGTACTGGATAGGCAGTAGTAGAAAAGAGCTTGGAAAGATGCCCGAGGATGTACAAGACGTATTCGG
TTACGCCCTGCATCTGGCTCAGGAAGGCAGCAAGCACCCTCAAGCGAAACCCCTTAAAGGGTTTTCTGGCGCGGGTGTTC
TGGAGGTTGTCGAAGACTTCGCAGGCGATACCTATCGGGCGGTTTACACCGTCAGATTCGCCAAGGTGGTCTATGCCCTC
CATTGCTTCCAGAAAAAGTCGAACAAAGGAATCGAGACGCCACAGCACGACATCGAACTGATCAAGGCACGACTGATCGC
TGCAGCAACGCATGCCAATGGAGTAGAGAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT139066 WP_002887278.1 NZ_CP043580:c4748158-4747892 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT139066 NZ_CP058349:1300938-1301258 [Pseudomonas sp. ABC1]
ATGACGGATATCGAAGAAAGCAGTGGCAATCCCTATGCCGATATTGGCCAAACCAATGCCAGTGAAATGCAGGTCAAGGC
AAGGCTGGCCACGAAGATCGGCGAAATCATCAAATCGCGGCGCCTGACCCAGCTACAGGCGGCAGAAATACTGGGACTCC
CACAGCCCAAGGTTTCCGACATGCTACGCGGGAAATTCAGGGGGATCAGCGAAGCCAAGATGATCGATTGCCTGTCCCGT
CTGGGGCGCGATGTGCAAATTCTGATCAAGACAGCGCCCCGCTCACGGAAAGAAGGACGGGTGGAAGTGATTTTCGCCTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References