Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4157910..4158529 Replicon chromosome
Accession NZ_CP043580
Organism Klebsiella pneumoniae strain SCKP020018

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag C6M74_RS20095 Protein ID WP_002892050.1
Coordinates 4158311..4158529 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag C6M74_RS20090 Protein ID WP_002892066.1
Coordinates 4157910..4158284 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M74_RS20080 (4153062) 4153062..4154255 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C6M74_RS20085 (4154278) 4154278..4157424 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
C6M74_RS20090 (4157910) 4157910..4158284 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
C6M74_RS20095 (4158311) 4158311..4158529 + 219 WP_002892050.1 HHA domain-containing protein Toxin
C6M74_RS20100 (4158688) 4158688..4159254 + 567 WP_002892030.1 maltose O-acetyltransferase -
C6M74_RS20105 (4159226) 4159226..4159366 - 141 WP_004147370.1 hypothetical protein -
C6M74_RS20110 (4159387) 4159387..4159857 + 471 WP_002892026.1 YlaC family protein -
C6M74_RS20115 (4159832) 4159832..4161283 - 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
C6M74_RS20120 (4161384) 4161384..4162082 + 699 WP_002892021.1 GNAT family protein -
C6M74_RS20125 (4162079) 4162079..4162219 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
C6M74_RS20130 (4162219) 4162219..4162482 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T139065 WP_002892050.1 NZ_CP043580:4158311-4158529 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T139065 NZ_CP058349:788709-789023 [Pseudomonas sp. ABC1]
ATGATTTTCATCGAAACTCCAATTTTCACCAAGCAGCTACGCGAGCTCTTGGATGACGATAGCTATGCAGCCTTCCAACG
CACCCTGGCTGATCGCCCAGAGCAAGGTGATGTTATCGAAGGCACGGGAGGCATTCGTAAAGTACGGGTGGCCTCCAGTG
GGCGCGGAAAGCGAGGTGGCTCCAGAGTTATCTACTACCACTTCTCATCGGCCTCACAGATCGCGCTACTGCTGATCTAC
CCAAAGAATGAAAAAGACGACTTGAGCACAGAGGAACGCAAAGCGCTCAAGCACATCATCGAACGGTGGAGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT139065 WP_002892066.1 NZ_CP043580:4157910-4158284 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT139065 NZ_CP058349:789029-789316 [Pseudomonas sp. ABC1]
ATGAGCAAATTTTTCGACGACCTTCTGGAGAGCGTCCAACAGATGGATGAAATCCATCGCGGCGAGAGGCAACCATCAAG
GGAGTTCACTGTAGACGCACTGCAGGTGAAGGAGATCCGTAAGGCAACTGGTCTGACTCAGGCCCGGTTCGCTGCAATGA
TCGACGTTCAGCTCGGCACTCTACGCAATTGGGAACAGGGACGCCGCGAACCAACAGGGCCAGCCAAAGCCTTGTTACGG
GCGATCCACAATGATCCAGCACACGTTATTCAGGCACTCGCGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References