Detailed information of TA system TA139053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 327566..328152
• Replicon: chromosome
• Accession: NZ_CP043580
• Organism: Klebsiella pneumoniae strain SCKP020018

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A330WAT7
• Locus tag: C6M74_RS01525
• Protein ID: WP_014906830.1
• Coordinates: 327784..328152 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: C6M74_RS01520
• Protein ID: WP_004174006.1
• Coordinates: 327566..327787 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6M74_RS01500 (323723)	323723..324649	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
C6M74_RS01505 (324646)	324646..325923	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
C6M74_RS01510 (325920)	325920..326687	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
C6M74_RS01515 (326689)	326689..327402	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
C6M74_RS01520 (327566)	327566..327787	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C6M74_RS01525 (327784)	327784..328152	+	369	WP_014906830.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C6M74_RS01530 (328425)	328425..329741	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
C6M74_RS01535 (329848)	329848..330735	+	888	WP_014906831.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
C6M74_RS01540 (330732)	330732..331577	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
C6M74_RS01545 (331579)	331579..332649	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	322552..333386	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (28-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13537.95 Da
• Isoelectric Point: 8.6410

>T139053 WP_014906830.1 NZ_CP043580:327784-328152 [Klebsiella pneumoniae]
MTLQIISAEEIILFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T139053 NZ_CP058343:75560-75958 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATAAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT139053 WP_004174006.1 NZ_CP043580:327566-327787 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT139053 NZ_CP058343:75333-75560 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A330WAT7

Antitoxin

• AlphaFold DB: A0A5E5YJY7