Detailed information of TA system TA139048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 5571057..5571940
• Replicon: chromosome
• Accession: NZ_CP043576
• Organism: Pseudomonas putida strain T25-27

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A173HDN1
• Locus tag: F0Q09_RS25475
• Protein ID: WP_019751987.1
• Coordinates: 5571057..5571494 (-)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: F0Q09_RS25480
• Protein ID: WP_182375194.1
• Coordinates: 5571491..5571940 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0Q09_RS25460	5567358..5568587	-	1230	WP_003250533.1	acyl-CoA dehydrogenase	-
F0Q09_RS25465	5568728..5569651	+	924	WP_004573723.1	LysR family transcriptional regulator	-
F0Q09_RS25470	5569845..5570999	+	1155	WP_004573724.1	aminotransferase class V-fold PLP-dependent enzyme	-
F0Q09_RS25475	5571057..5571494	-	438	WP_019751987.1	RES family NAD+ phosphorylase	Toxin
F0Q09_RS25480	5571491..5571940	-	450	WP_182375194.1	DUF2384 domain-containing protein	Antitoxin
F0Q09_RS25485	5572083..5572736	-	654	WP_004573726.1	oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB	-
F0Q09_RS25490	5572840..5573763	+	924	WP_004573727.1	LysR family transcriptional regulator	-
F0Q09_RS25495	5573812..5574642	+	831	WP_003250518.1	AraC family transcriptional regulator	-
F0Q09_RS25500	5574694..5575278	+	585	WP_004573728.1	LysE family transporter	-
F0Q09_RS25505	5575318..5576517	-	1200	WP_003250514.1	sugar transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-138)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (33-91)

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15953.22 Da
• Isoelectric Point: 6.0704

>T139048 WP_019751987.1 NZ_CP043576:c5571494-5571057 [Pseudomonas putida]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPPGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSAHPELTRTLGNRFLDDGSALLLPVPSAIMPSTTNYLFNPRHPQAQSAKIQVEDFTPDSRLF

Nucleotide

• Length: 438 bp

>T139048 NZ_CP058342:3753479-3753757 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16942.43 Da
• Isoelectric Point: 5.7258

>AT139048 WP_182375194.1 NZ_CP043576:c5571940-5571491 [Pseudomonas putida]
MLAEVLRDNGYHEYRARLQALLDIPELASDFEIHTRITDGFAATWLVKLTERGVLSPVERDQIIPLRTLKSRIERDQPLT
VDESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGYGL

Nucleotide

• Length: 450 bp

>AT139048 NZ_CP058342:3753216-3753476 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A173HDN1