Detailed information of TA system TA139047

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3665509..3666548
• Replicon: chromosome
• Accession: NZ_CP043576
• Organism: Pseudomonas putida strain T25-27

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: F0Q09_RS16560
• Protein ID: WP_019750859.1
• Coordinates: 3665509..3666084 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: A0A173HI04
• Locus tag: F0Q09_RS16565
• Protein ID: WP_003251469.1
• Coordinates: 3666081..3666548 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0Q09_RS16550	3661126..3662361	+	1236	WP_108079141.1	FAD-dependent oxidoreductase	-
F0Q09_RS16555	3662365..3665295	-	2931	WP_182374805.1	aminotransferase	-
F0Q09_RS16560	3665509..3666084	-	576	WP_019750859.1	PIN domain-containing protein	Toxin
F0Q09_RS16565	3666081..3666548	-	468	WP_003251469.1	helix-turn-helix domain-containing protein	Antitoxin
F0Q09_RS16570	3666693..3668300	-	1608	WP_070095848.1	ABC transporter ATP-binding protein	-
F0Q09_RS16575	3668302..3669321	-	1020	WP_010954919.1	ABC transporter permease	-
F0Q09_RS16580	3669323..3670396	-	1074	WP_003251466.1	microcin C ABC transporter permease YejB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21683.92 Da
• Isoelectric Point: 5.5710

>T139047 WP_019750859.1 NZ_CP043576:c3666084-3665509 [Pseudomonas putida]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFPKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHNVQQYLDRLLQQKLPETVKLLSAFKFLL

Nucleotide

• Length: 576 bp

>T139047 NZ_CP058342:c3747417-3747019 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17229.71 Da
• Isoelectric Point: 6.6505

>AT139047 WP_003251469.1 NZ_CP043576:c3666548-3666081 [Pseudomonas putida]
MTTANLSRHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRTAMDALAAQAQELKMGYE

Nucleotide

• Length: 468 bp

>AT139047 NZ_CP058342:c3747713-3747420 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A173HI04