Detailed information of TA system TA139045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2728810..2729396
• Replicon: chromosome
• Accession: NZ_CP043576
• Organism: Pseudomonas putida strain T25-27

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: F0Q09_RS12415
• Protein ID: WP_082423705.1
• Coordinates: 2728810..2729103 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: I3UY28
• Locus tag: F0Q09_RS12420
• Protein ID: WP_004576347.1
• Coordinates: 2729100..2729396 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0Q09_RS12390	2724411..2725265	+	855	WP_060538644.1	putative selenate ABC transporter substrate-binding protein	-
F0Q09_RS12395	2725262..2726071	+	810	WP_182375545.1	phosphonate ABC transporter ATP-binding protein	-
F0Q09_RS12400	2726065..2726892	+	828	WP_003248575.1	ABC transporter permease subunit	-
F0Q09_RS12405	2726889..2727656	+	768	WP_003248573.1	phosphonate ABC transporter, permease protein PhnE	-
F0Q09_RS12410	2727693..2728586	-	894	WP_003248571.1	hypothetical protein	-
F0Q09_RS12415	2728810..2729103	+	294	WP_082423705.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F0Q09_RS12420	2729100..2729396	+	297	WP_004576347.1	putative addiction module antidote protein	Antitoxin
F0Q09_RS12425	2729407..2729886	-	480	WP_004576346.1	GNAT family N-acetyltransferase	-
F0Q09_RS12430	2730057..2730704	-	648	WP_064615263.1	serine protease	-
F0Q09_RS12440	2731157..2732206	+	1050	WP_182375546.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
F0Q09_RS12445	2732221..2733336	+	1116	WP_003248558.1	tRNA guanosine(34) transglycosylase Tgt	-
F0Q09_RS12450	2733379..2733714	+	336	WP_003248556.1	preprotein translocase subunit YajC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-96)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10799.60 Da
• Isoelectric Point: 10.8302

>T139045 WP_082423705.1 NZ_CP043576:2728810-2729103 [Pseudomonas putida]
MFLVEQTESFAAWLSSLRDLRARLAIGRRLERAAAGNLGDFKWLGGGIGELRIDVAAGYRFYFTQKGQRLVLLLVGGDKS
TQAADILNARKLLKEMK

Nucleotide

• Length: 294 bp

>T139045 NZ_CP058342:2719857-2719964 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10676.33 Da
• Isoelectric Point: 6.7249

>AT139045 WP_004576347.1 NZ_CP043576:2729100-2729396 [Pseudomonas putida]
MSEKLIPFDMAALLSSDEAISEYLSQVLSNGDTEEIIRALGHIARARGMTHIATESGLGRESLYKALSPGAKPRFDTVLR
VIRALGVDLLVQPHAMTR

Nucleotide

• Length: 297 bp

>AT139045 NZ_CP043576:2729100-2729396 [Pseudomonas putida]
ATGAGCGAAAAATTGATCCCGTTTGATATGGCAGCCTTGTTGAGCAGCGATGAGGCAATCAGTGAATACCTTAGCCAGGT
GCTATCAAATGGCGATACCGAGGAAATCATTCGAGCACTTGGCCATATCGCTCGTGCACGGGGCATGACACATATTGCTA
CAGAGAGTGGGCTGGGTAGAGAAAGCCTGTACAAGGCGCTGAGCCCTGGCGCGAAGCCTCGTTTTGACACTGTATTGAGG
GTCATTCGTGCTCTGGGGGTGGACCTTCTCGTTCAGCCACATGCAATGACCCGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2I0CD26