Detailed information of TA system TA139044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/YoeB-YefM
• Location: 566307..566809
• Replicon: chromosome
• Accession: NZ_CP043575
• Organism: Comamonas koreensis strain T50-37

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q5XQD8
• Locus tag: F0Q04_RS02645
• Protein ID: WP_032492620.1
• Coordinates: 566307..566561 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0BEH6
• Locus tag: F0Q04_RS02650
• Protein ID: WP_032492621.1
• Coordinates: 566558..566809 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0Q04_RS02605	561318..561653	+	336	WP_182344325.1	hypothetical protein	-
F0Q04_RS02610	561680..562642	+	963	WP_182344327.1	hypothetical protein	-
F0Q04_RS02615	562663..562959	+	297	WP_182283343.1	H-NS histone family protein	-
F0Q04_RS02620	563032..563313	+	282	WP_182283342.1	hypothetical protein	-
F0Q04_RS02625	563335..563508	+	174	WP_182283341.1	hypothetical protein	-
F0Q04_RS02630	563965..564669	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
F0Q04_RS02635	564722..565519	-	798	Protein_514	class 1 integron integrase IntI1	-
F0Q04_RS02640	565680..566234	+	555	WP_003159191.1	aminoglycoside N-acetyltransferase AAC(6')-Ib4	-
F0Q04_RS02645	566307..566561	-	255	WP_032492620.1	Txe/YoeB family addiction module toxin	Toxin
F0Q04_RS02650	566558..566809	-	252	WP_032492621.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
F0Q04_RS02655	566983..567537	+	555	WP_014454105.1	aminoglycoside N-acetyltransferase AAC(6')-Ib'	-
F0Q04_RS02660	567642..568894	+	1253	Protein_519	EreA family erythromycin esterase	-
F0Q04_RS02665	568992..569819	+	828	WP_014386432.1	OXA-2 family oxacillin-hydrolyzing class D beta-lactamase OXA-21	-
F0Q04_RS02670	569867..570340	+	474	WP_071846295.1	trimethoprim-resistant dihydrofolate reductase DfrA1	-
F0Q04_RS02675	570532..570879	+	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	aac(6')-Ib / ere(A) / blaOXA-21 / floR / aph(3'')-Ib / aph(6)-Id / tet(G) / sul1 / aph(3')-Ib / ant(3'')-Ia	-	561006..604101	43095
	-	inside	IScluster/Tn	aac(6')-Ib / ere(A) / blaOXA-21 / floR / aph(3'')-Ib / aph(6)-Id / tet(G) / sul1 / aph(3')-Ib / ant(3'')-Ia	-	559420..601734	42314
	-	inside	Integron	-	-	564560..568894	4334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10272.81 Da
• Isoelectric Point: 9.3585

>T139044 WP_032492620.1 NZ_CP043575:c566561-566307 [Comamonas koreensis]
MRLIFSDDAWEDYLFWQKQDRRMVDRINKLIKETTREPFSGVGKPEPLKHALAGYWSRRITDEHRMVYKVADDALWIVQL
KYHY

Nucleotide

• Length: 255 bp

>T139044 NZ_CP058342:2719322-2719429 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9244.40 Da
• Isoelectric Point: 4.7769

>AT139044 WP_032492621.1 NZ_CP043575:c566809-566558 [Comamonas koreensis]
MDAITYTHARANLAGTMDRVCNDHEPVIITRNGDQSVVILSLEDFQALEETAYLLRSPANAKRLFKSIEQLEAGRGQARE
LAE

Nucleotide

• Length: 252 bp

>AT139044 NZ_CP058342:c2719275-2719209 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A1V0BEH9

Antitoxin

• AlphaFold DB: A0A1V0BEH6