Detailed information of TA system TA139043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 424274..425896
• Replicon: chromosome
• Accession: NZ_CP043575
• Organism: Comamonas koreensis strain T50-37

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: F0Q04_RS02050
• Protein ID: WP_182345525.1
• Coordinates: 424274..425584 (-)
• Length: 437 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: F0Q04_RS02055
• Protein ID: WP_116927469.1
• Coordinates: 425627..425896 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0Q04_RS02025	419606..420967	+	1362	WP_182344202.1	glycerophosphodiester phosphodiesterase	-
F0Q04_RS02030	421025..421714	-	690	WP_182344204.1	amino acid ABC transporter permease	-
F0Q04_RS02035	421711..422478	-	768	WP_182344206.1	amino acid ABC transporter permease	-
F0Q04_RS02040	422547..423371	-	825	WP_116927471.1	ABC transporter substrate-binding protein	-
F0Q04_RS02045	423622..424227	+	606	WP_182344208.1	hypothetical protein	-
F0Q04_RS02050	424274..425584	-	1311	WP_182345525.1	type II toxin-antitoxin system HipA family toxin	Toxin
F0Q04_RS02055	425627..425896	-	270	WP_116927469.1	helix-turn-helix domain-containing protein	Antitoxin
F0Q04_RS02060	425997..428750	-	2754	WP_182344210.1	methionine synthase	-
F0Q04_RS02065	429124..430362	+	1239	WP_182344212.1	sodium/glutamate symporter	-
F0Q04_RS02070	430427..430846	-	420	WP_027010524.1	PACE efflux transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (1-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-67)

Sequences

Toxin

Protein

• Length: 437 a.a.
• Molecular weight: 48587.69 Da
• Isoelectric Point: 6.2449

>T139043 WP_182345525.1 NZ_CP043575:c425584-424274 [Comamonas koreensis]
MNGHRVGTWRVEAGSDVLQYDDAWVRDVALRRPLSLSLPFTPGNGPHKGEAVRFYFQNLLPDNEQILERIARRYKVGATD
PNALLREIGRDCVGALQILPAGEASPEEPEMQYEVLSEADVARLVRAVVNPTQSGDGGLDDDDFRISIAGAQEKTALLYW
AGQWCRPLGATPTSHILKLPLGLIGNMQIDMRHSVENEWLCSKLVAAFGIPIAPCDVLRFEDQKVLAVERFDRSVWKEKV
LVRIPQEDMCQASGVSPILKYESDGGPGVDTIMDLLARSKSPGTDRFQFFMAQLLFWMLCATDGHAKNFSIFLRPQGVFE
MTPLYDVLSAYPIWGAGPGKLSPHKARMAMAVRSKNAHWNMNKIMRCHWEAVGERYGIRSPQGLDVRAIIDQVVEMTPDV
IARAEKQLPPDFPQSVSTAIFQGLQDAANRLGRVHD

Nucleotide

• Length: 1311 bp

>T139043 NZ_CP058342:c2506360-2506184 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9805.32 Da
• Isoelectric Point: 10.2947

>AT139043 WP_116927469.1 NZ_CP043575:c425896-425627 [Comamonas koreensis]
MDEFTVRTTAQLPTLLKAFRKTAKLTQADVATRLGVTQQTVSAMERNAESVSAERLMKLMGILGVDLVLRARPATSPDTG
RELQTLDSW

Nucleotide

• Length: 270 bp

>AT139043 NZ_CP058342:c2506138-2505722 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA