Detailed information of TA system TA139042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 77921..78404
• Replicon: plasmid unnamed
• Accession: NZ_CP043574
• Organism: Comamonas testosteroni strain R5-28

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: F0Q02_RS26715
• Protein ID: WP_174863007.1
• Coordinates: 78132..78404 (+)
• Length: 91 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: F0Q02_RS26710
• Protein ID: WP_182312697.1
• Coordinates: 77921..78148 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0Q02_RS26675	73328..73675	-	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-
F0Q02_RS26680	73832..74464	-	633	WP_000186237.1	type B-3 chloramphenicol O-acetyltransferase CatB3	-
F0Q02_RS26685	74648..74899	+	252	WP_032492621.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
F0Q02_RS26690	74896..75150	+	255	WP_032492620.1	Txe/YoeB family addiction module toxin	-
F0Q02_RS26695	75325..75879	-	555	WP_071846204.1	aminoglycoside 6'-N-acetyltransferase	-
F0Q02_RS26700	75962..76201	-	240	WP_165720489.1	DfrB family trimethoprim-resistant dihydrofolate reductase	-
F0Q02_RS26705	76462..77475	+	1014	WP_000845048.1	class 1 integron integrase IntI1	-
F0Q02_RS26710	77921..78148	+	228	WP_182312697.1	TraY domain-containing protein	Antitoxin
F0Q02_RS26715	78132..78404	+	273	WP_174863007.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
F0Q02_RS26720	78437..78640	-	204	WP_174863008.1	hypothetical protein	-
F0Q02_RS26725	78631..79218	-	588	WP_182312700.1	cobyrinic acid ac-diamide synthase	-
F0Q02_RS26735	80935..81171	-	237	WP_100245271.1	hypothetical protein	-
F0Q02_RS26740	82235..83140	+	906	WP_000219391.1	Mph(A) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1 / tet(G) / aph(6)-Id / aph(3'')-Ib / qacE / catB3 / aac(6')-IIc / dfrB7 / mph(A)	-	1..248727	248727
	-	inside	IScluster/Tn	sul1 / tet(G) / aph(6)-Id / aph(3'')-Ib / qacE / catB3 / aac(6')-IIc / dfrB7 / mph(A)	-	49794..90457	40663
	-	inside	Integron	-	-	73328..77475	4147

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Relaxosome protein TraY (3-49)

Sequences

Toxin

Protein

• Length: 91 a.a.
• Molecular weight: 10339.97 Da
• Isoelectric Point: 10.2187

>T139042 WP_174863007.1 NZ_CP043574:78132-78404 [Comamonas testosteroni]
MAWQIELSEGAVKNLKQLDHQHARRILSFLHGRVAQLDDPRSIGEALKGSKLGELWKYRVGDYRIIADIEDGVMRVLVLK
VGNRREVYRG

Nucleotide

• Length: 273 bp

>T139042 NZ_CP058342:2419199-2420521 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8557.80 Da
• Isoelectric Point: 5.1023

>AT139042 WP_182312697.1 NZ_CP043574:77921-78148 [Comamonas testosteroni]
MSVSLRLPPELNQRLSHLAEQTGRSKTFYMLEAIKQHLDDLEDVYLAERELEAVRAGKSKAVPLEEVMKQYGLAD

Nucleotide

• Length: 228 bp

>AT139042 NZ_CP058342:2418933-2419199 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA